Naderi Ghazal, Asadian Mahla, Seifi Arash, Ghourchian Sedigheh, Talebi Malihe, Rahbar Mohammad, Abdollahi Alireza, Douraghi Masoumeh
Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences , Tehran, Iran.
Department of Infectious Diseases, Faculty of Medicine, Tehran University of Medical Sciences , Tehran, Iran.
Microbiol Spectr. 2023 Aug 28;11(5):e0537322. doi: 10.1128/spectrum.05373-22.
strains belonging to global clone 2 (GC2) contain resistance islands (AbGRIs), which are composed of genes conferring resistance to older and newer antibiotics. Here, to locate these genes in AbGRIs, the GC2 strains from Tehran, Iran were examined. Among the 170 . , 90 isolates were identified as GC2. Of the genes that confer resistance to older antibiotics, ), (tetracyclines), , and (aminoglycosides) were located in AbGRI1 of 65 GC2 isolates (72.2%). Of the other aminoglycosides, the was located in AbGRI2-12b (63.6%), AbGRI2-12a (21.2%), or AbGRI2-1 (15.1%). The and genes were co-located within AbGRI2-1 (5.5%). The was located in AbGRI3-4 (77.7%) and AbGRI3 (22.2%). Of sulfonamides, the was located within AbGRI2-1 (5.5%). Of beta-lactams, the was located in AbGRI2-12b (42%), AbGRI2-12a (14%), AbGRI2-1 (10%), or AbGRI2 (34%). The gene conferring resistance to newer antibiotics (carbapenems) was located in AbaR4 (81.1%); of them, the AbaR4 was located within AbGRI1 in 45.2% of the isolates. This study showed that the GC2 isolates, which contained at least one AbGRI, disseminate in the hospital. Hence, it is likely that the AbGRIs play a significant role in conferring resistance to older and newer antibiotics in GC2 isolates from Iran. IMPORTANCE The majority of isolates that are resistant to multiple antibiotics belong to one of the two major global clones, namely global clone 1 (GC1) and global clone 2 (GC2). The resistance islands, which contain variable assortments of transposons, integrons, and specific resistance genes, have been characterized in the genome of these GCs. In GC2 the chromosomally located genomic resistance islands (AbGRIs) carry the genes conferring resistance to older and newer antibiotics. In this context, we tested whether GC2 isolates collected from a referral hospital carry the AbGRIs containing these genes. This study provided evidence for the circulation of the GC2 strains harboring AbGRI resistance islands between different wards of a referral hospital.
属于全球克隆2（GC2）的菌株含有耐药岛（AbGRIs），这些耐药岛由赋予对新旧抗生素耐药性的基因组成。在此，为了在AbGRIs中定位这些基因，对来自伊朗德黑兰的GC2菌株进行了检测。在170株……中，90株被鉴定为GC2。在赋予对旧抗生素耐药性的基因中，……（四环素类）、……和……（氨基糖苷类）位于65株GC2分离株（72.2%）的AbGRI1中。在其他氨基糖苷类中，……位于AbGRI2 - 12b（63.6%）、AbGRI2 - 12a（21.2%）或AbGRI2 - 1（15.1%）中。……和……基因共定位在AbGRI2 - 1内（5.5%）。……位于AbGRI3 - 4（77.7%）和AbGRI3（22.2%）中。在磺胺类中，……位于AbGRI2 - 1内（5.5%）。在β - 内酰胺类中，……位于AbGRI2 - 12b（42%）、AbGRI2 - 12a（14%）、AbGRI2 - 1（10%）或AbGRI2（34%）中。赋予对新抗生素（碳青霉烯类）耐药性的……基因位于AbaR4中（81.1%）；其中，45.2%的分离株中AbaR4位于AbGRI1内。本研究表明，含有至少一个AbGRI的GC2分离株在医院中传播。因此，AbGRIs很可能在赋予伊朗GC2分离株对新旧抗生素的耐药性方面发挥重要作用。重要性 大多数对多种抗生素耐药的……分离株属于两个主要全球克隆之一，即全球克隆1（GC1）和全球克隆2（GC2）。在这些GCs的基因组中已经对含有转座子、整合子和特定耐药基因的可变组合的耐药岛进行了表征。在GC2中，位于染色体上的……基因组耐药岛（AbGRIs）携带赋予对新旧抗生素耐药性的基因。在这种情况下，我们测试了从一家转诊医院收集的GC2分离株是否携带含有这些基因的AbGRIs。本研究为携带AbGRI耐药岛的GC2菌株在一家转诊医院的不同病房之间传播提供了证据。
