Institute for Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna, Vienna, Austria.
Aix Marseille Univ, CNRS, INSERM, CIML, Centre d'Immunologie de Marseille-Luminy, Marseille, France.
Methods Mol Biol. 2024;2713:363-376. doi: 10.1007/978-1-0716-3437-0_25.
Functional reprograming of cells is linked to a process of metabolic rewiring that is adapted for such new functions or microenvironment. Macrophages are present in all tissues and exposed to different microenvironments throughout our body. Profiling energetic metabolism of tissue resident and other heterogeneous populations of macrophages in vitro and ex vivo is technologically very challenging. We have recently developed a method to functionally profile energetic metabolism with single-cell resolution, named SCENITH. This method can be performed rapidly ex vivo and does not require specialized equipment. In this book chapter, we will summarize the tissue processing, the procedure and methods, the analysis and example of results, and a series of frequently asked questions.
细胞的功能重编程与代谢重排过程相关，该过程适应于新功能或微环境。巨噬细胞存在于所有组织中，并在我们体内的不同微环境中暴露。对组织驻留细胞和其他异质巨噬细胞群体的体外和离体能量代谢进行分析是一项极具技术挑战性的工作。我们最近开发了一种具有单细胞分辨率的能量代谢功能分析方法，称为 SCENITH。这种方法可以在体外快速进行，并且不需要特殊的设备。在本章中，我们将总结组织处理、程序和方法、分析和结果示例以及一系列常见问题。
