Department of Microbiology & Immunology, University of California , San Francisco, California, USA.
Biological Sciences Group, Pacific Northwest National Laboratory , Richland, Washington, USA.
mBio. 2023 Oct 31;14(5):e0157323. doi: 10.1128/mbio.01573-23. Epub 2023 Aug 29.
This work has broad relevance due to the ubiquity of dyes containing azo bonds in food and drugs. We report that azo dyes can be degraded by human gut bacteria through both enzymatic and nonenzymatic mechanisms, even from a single gut bacterial species. Furthermore, we revealed that environmental factors, oxygen, and L-Cysteine control the ability of to degrade azo dyes due to their impacts on bacterial transcription and metabolism. These results open up new opportunities to manipulate the azoreductase activity of the gut microbiome through the manipulation of host diet, suggest that azoreductase potential may be altered in patients suffering from gastrointestinal disease, and highlight the importance of studying bacterial enzymes for drug metabolism in their natural cellular and ecological context.
由于食品和药物中含有偶氮键的染料无处不在，这项工作具有广泛的相关性。我们报告说，偶氮染料可以通过人类肠道细菌的酶促和非酶促机制进行降解，甚至可以从单个肠道细菌物种中进行降解。此外，我们揭示了环境因素、氧气和 L-半胱氨酸通过影响细菌转录和代谢来控制的能力，从而降解偶氮染料。这些结果为通过操纵宿主饮食来操纵肠道微生物组的偶氮还原酶活性开辟了新的机会，表明患有胃肠道疾病的患者的偶氮还原酶潜力可能会发生改变，并强调了在其自然细胞和生态环境中研究细菌酶用于药物代谢的重要性。
