Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China.
School of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo 255049, P. R. China.
Anal Chem. 2023 Sep 12;95(36):13596-13604. doi: 10.1021/acs.analchem.3c02289. Epub 2023 Aug 29.
Metal oxide nanomaterials have garnered significant attention in the field of electrochemiluminescence (ECL) sensing due to their efficient, stable, and nontoxic properties. However, the current research on metal oxide nanomaterials has primarily focused on their cathodic luminescence properties, with limited reports on their anodic ECL properties. In this study, we utilized MOF-derived ZrO nanomaterials as luminophores to generate stable anodic ECL signals in the presence of the coreactant tripropylamine (TPrA). Additionally, a signal-enhancing immunosensor was developed to analyze D-dimer by incorporating the coreaction accelerator Cu-doped TiO (TiO-Cu). The ZrO synthesized by calcining UiO-67 demonstrated nontoxicity and biocompatibility, exhibiting efficient and stable ECL emission in a TPrA solution. The inclusion of TiO-Cu as a coreaction accelerator in the immunosensor resulted in the formation of a ternary system of ZrO/TiO-Cu/TPrA. The Cu doping effectively narrowed the bandgap of TiO and enhanced its conductivity. As a substrate, TiO-Cu reacted with more TPrA, generating sufficient free radicals to effectively enhance the ECL signal of ZrO. In this article, a short peptide ligand, NFC (NARKFYKGC), was designed to immobilize antibodies and maintain the activity of antigen-binding sites during the construction of the immunosensor. The developed immunosensor was used for the accurate detection of D-dimers, with a wide linear range of 0.05-600 ng/mL and a low detection limit of 21 pg/mL..
金属氧化物纳米材料因其高效、稳定和无毒的特性，在电致化学发光（ECL）传感领域引起了广泛关注。然而，目前对金属氧化物纳米材料的研究主要集中在其阴极发光性质上，对其阳极 ECL 性质的报道有限。在本研究中，我们利用 MOF 衍生的 ZrO 纳米材料作为发光体，在共反应物三丙胺（TPrA）存在下产生稳定的阳极 ECL 信号。此外，通过掺入共反应加速剂 Cu 掺杂的 TiO（TiO-Cu），开发了一种信号增强的免疫传感器来分析 D-二聚体。由 UiO-67 煅烧得到的 ZrO 表现出无毒和生物相容性，在 TPrA 溶液中表现出高效和稳定的 ECL 发射。将 TiO-Cu 作为共反应加速剂纳入免疫传感器中，形成了 ZrO/TiO-Cu/TPrA 的三元体系。Cu 掺杂有效地缩小了 TiO 的带隙并提高了其导电性。作为基底，TiO-Cu 与更多的 TPrA 反应，生成足够的自由基，有效地增强了 ZrO 的 ECL 信号。在本文中，设计了一个短肽配体 NFC（NARKFYKGC），用于固定抗体并在免疫传感器的构建过程中保持抗原结合位点的活性。所开发的免疫传感器可用于准确检测 D-二聚体，线性范围为 0.05-600 ng/mL，检测限低至 21 pg/mL。
