College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Theriogenology. 2023 Nov;211:172-181. doi: 10.1016/j.theriogenology.2023.08.017. Epub 2023 Aug 23.
Bromodomain PHD finger transcription factor (BPTF), a core subunit of nucleosome-remodeling factor (NURF) complex, plays an important role in chromatin remodeling. However, few information of BPTF is available in pig, especially in mammalian follicular granulosa cells (GCs). The present study firstly confirmed that BPTF in porcine was relative close to human and mouse. The expression of BPTF could be detected in ovary, testes, lung, kidney, large intestine, and small intestine. And a relative high expression of BPTF was observed in ovarian follicles and GCs. When BPTF was knocked down (BPTF-siRNA), the viability of GCs was affected. And the expression level of CDK1, cyclin B1, CDK4 and CDK2 was higher than the control, which might indicate that the cell cycle of GCs was inhibited from S to G2/M phase. Although the apoptosis level was induced in the BPTF-siRNA GCs, the reduced level of H3K4 methylation was detected with the down regulation of SMYD3, EHMT2 and DPY30. Thereby, results in the present might provide the primary knowledge of BPTF in GCs and the follicular development in pig.
溴结构域和 PH 结构域家族转录因子（Bromodomain PHD finger transcription factor，BPTF）是核小体重塑因子（nucleosome-remodeling factor，NURF）复合物的核心亚基，在染色质重塑中发挥重要作用。然而，关于猪 BPTF 的信息很少，特别是在哺乳动物卵泡颗粒细胞（follicular granulosa cells，GCs）中。本研究首次证实猪 BPTF 与人和鼠相对接近。BPTF 在卵巢、睾丸、肺、肾、大肠和小肠中均有表达，在卵巢卵泡和 GCs 中表达水平较高。当 BPTF 被敲低（BPTF-siRNA）时，GCs 的活力受到影响。并且 CDK1、cyclin B1、CDK4 和 CDK2 的表达水平高于对照组，这可能表明 GCs 的细胞周期从 S 期被抑制到 G2/M 期。尽管 BPTF-siRNA GCs 中诱导了凋亡水平，但随着 SMYD3、EHMT2 和 DPY30 的下调，检测到 H3K4 甲基化水平降低。因此，本研究结果可能为 GCs 中的 BPTF 以及猪卵泡发育提供了初步知识。
