Department of Paramedical and Allied Health Sciences, Midnapore City College, Midnapore, 721129, West Bengal, India.
Department of Food Chemistry and Nutrient Analysis, National Institute of Nutrition (NIN), Hyderabad, Telengana, 500007, India.
Sci Rep. 2023 Aug 29;13(1):14125. doi: 10.1038/s41598-023-34885-3.
The implication of inflammation in the pathophysiology of several types of cancers has been under intense investigation. Conjugated fatty acids can modulate inflammation and present anticancer effects, promoting cancer cell death. In this paper, we evaluated the efficacy of new conjugated fatty acids isolated from marine Opisthopterus tardoore (Tapra fish) in human breast cancer cell lines MCF-7. Linoelaidic acid, a marine fish (O. tardoore) derived unsaturated fatty acids, showed effective anticancer activity against MCF-7. Cell viability (MTT) assay revealed a dose-dependent decline in cancer cell viability. It was noteworthy that 5 µM linoelaidic acid decreased the MCF-7 cell viability by 81.82%. Besides that, linoelaidic acid significantly (P< 0.05) increased the level of tumor necrosis factor-α (TNF-α) and interleukin-1 receptor antagonist (IL-1ra) studied by ELISA. Not only that, linoelaidic acid significantly decreased the reduced glutathione level and increased the oxidized glutathione level in MCF-7 cells indicating the oxidative stress inside the cell. Two different cell staining methods with acridine orange-ethidium bromide and DAPI confirmed that the linoelaidic acid rendered their detrimental effect on cancer cells. To decipher the mode of apoptosis Western blotting was performed in which the expression pattern of several proteins (p53, IL-10, and IL-1ra) established the apoptosis in the studied cell lines after linoelaidic acid exposure. Hence it may be conferred that linoelaidic acid has prompt anticancer activity. Therefore this drug can be used further for the treatment of cancer.
炎症在几种类型癌症的病理生理学中的作用一直受到深入研究。共轭脂肪酸可以调节炎症并表现出抗癌作用，促进癌细胞死亡。在本文中，我们评估了从海洋 Opisthopterus tardoore（Tapra 鱼）中分离出的新型共轭脂肪酸对人乳腺癌 MCF-7 细胞系的功效。亚油酸，一种海洋鱼类（O. tardoore）衍生的不饱和脂肪酸，对 MCF-7 表现出有效的抗癌活性。细胞活力（MTT）测定显示癌细胞活力呈剂量依赖性下降。值得注意的是，5 μM 亚油酸使 MCF-7 细胞活力降低了 81.82%。除此之外，亚油酸显著（P<0.05）增加了通过 ELISA 研究的肿瘤坏死因子-α（TNF-α）和白细胞介素-1 受体拮抗剂（IL-1ra）的水平。不仅如此，亚油酸显著降低了 MCF-7 细胞中的还原型谷胱甘肽水平并增加了氧化型谷胱甘肽水平，表明细胞内存在氧化应激。吖啶橙-溴化乙锭和 DAPI 的两种不同细胞染色方法证实，亚油酸对癌细胞产生了有害影响。为了解细胞凋亡的模式，进行了 Western blot 分析，其中几种蛋白质（p53、IL-10 和 IL-1ra）的表达模式在亚油酸暴露后确立了研究细胞系中的细胞凋亡。因此，可以认为亚油酸具有迅速的抗癌活性。因此，这种药物可进一步用于癌症的治疗。
