Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou, China.
Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Fujian Medical University, Fuzhou, China.
J Clin Lab Anal. 2019 Jul;33(6):e22903. doi: 10.1002/jcla.22903. Epub 2019 May 17.
Interleukin-1 promotes tumor angiogenesis through VEGF production. The interleukin-1 receptor antagonist can suppress tumors by blocking this effect.
Immunohistochemistry, WB, and gene sequencing were used to analyze the expression of IL-1RA in esophageal cancer patients. WB was used to detect the expression of IL-1RA and interleukin-1α in esophageal cancer cells. Stable ESCC cell models overexpressing the IL-1RA were constructed. Their cell functions were tested, and their effects on VEGF were examined.
IL-1RA is downregulated in primary EC tumors, and this downregulation of IL-1RA is closely related to TNM staging and survival prognosis. The overexpression of IL-1RA increased the proliferation of KYSE410 EC cells, which have a high level of IL-1α expression. Overexpression of IL-1RA in KYSE410 cells promotes a decrease in the expression of VEGF-A. However, IL-1RA expression did not cause any changes in EC9706 cells with low IL-1α expression.
IL-1RA acts as a tumor suppressor, and its deletion promotes tumor progression by increasing VEGF-A expression in ESCC.
白细胞介素-1 通过促进血管内皮生长因子（VEGF）的产生来促进肿瘤血管生成。白细胞介素-1 受体拮抗剂可通过阻断这种作用来抑制肿瘤。
采用免疫组织化学、WB 和基因测序分析食管癌患者中 IL-1RA 的表达。采用 WB 检测食管癌细胞中 IL-1RA 和白细胞介素-1α 的表达。构建 IL-1RA 过表达的稳定 ESCC 细胞模型。测试其细胞功能，并检测其对 VEGF 的影响。
IL-1RA 在原发性 EC 肿瘤中表达下调，IL-1RA 的下调与 TNM 分期和生存预后密切相关。IL-1RA 的过表达增加了 IL-1α 表达水平较高的 KYSE410 EC 细胞的增殖。在 KYSE410 细胞中过表达 IL-1RA 会导致 VEGF-A 的表达降低。然而，在 IL-1α 表达水平较低的 EC9706 细胞中，IL-1RA 的表达并未引起任何变化。
IL-1RA 作为一种肿瘤抑制因子，其缺失通过增加 ESCC 中 VEGF-A 的表达促进肿瘤进展。
