Peng Ruonan, Chen Xinye, Xu Fengjun, Hailstone Richard, Men Yujie, Du Ke
Department of Chemical and Environmental Engineering, University of California, Riverside, 900 University Ave, Riverside, CA 92507, USA.
Department of Microsystems Engineering, Rochester Institute of Technology, 1 Lomb Memorial Dr, Rochester, NY 14623, USA.
bioRxiv. 2023 Aug 18:2023.08.17.553737. doi: 10.1101/2023.08.17.553737.
The increasing prevalence of antibiotic-resistant bacterial infections, particularly methicillin-resistant Staphylococcus aureus (MRSA), presents a significant public health concern. Timely detection of MRSA is crucial to enable prompt medical intervention, limit its spread, and reduce antimicrobial resistance. Here, we introduce a miniaturized nano-sieve device featuring a pneumatically-regulated chamber for highly efficient MRSA purification from human plasma samples. By using packed magnetic beads as a filter and leveraging the deformability of the nano-sieve channel, we achieve an on-chip concentration factor of 15 for MRSA. We integrated this device with recombinase polymerase amplification (RPA) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas detection system, resulting in an on-chip limit of detection (LOD) of approximately 100 CFU/mL. This developed approach provides a rapid, precise, and centrifuge-free solution suitable for point-of-care diagnostics, with the potential to significantly improve patient outcomes in resource-limited medical conditions.
抗生素耐药性细菌感染，尤其是耐甲氧西林金黄色葡萄球菌（MRSA）的日益流行，引起了重大的公共卫生关注。及时检测MRSA对于及时进行医疗干预、限制其传播以及减少抗菌药物耐药性至关重要。在此，我们介绍一种小型化的纳米筛装置，其具有一个气动调节腔室，用于从人血浆样本中高效纯化MRSA。通过使用填充磁珠作为过滤器并利用纳米筛通道的可变形性，我们实现了MRSA在芯片上15倍的浓缩系数。我们将该装置与重组酶聚合酶扩增（RPA）和规律成簇间隔短回文重复序列（CRISPR）-Cas检测系统集成，在芯片上实现了约100 CFU/mL的检测限（LOD）。这种开发的方法提供了一种快速、精确且无需离心的解决方案，适用于即时诊断，有可能在资源有限的医疗条件下显著改善患者的治疗效果。
