Department of Chemical and Environmental Engineering, University of California, Riverside, 900 University Ave, Riverside, CA 92507, USA.
Department of Microsystems Engineering, Rochester Institute of Technology, 1 Lomb Memorial Dr, Rochester, NY 14623, USA.
Nanoscale Horiz. 2023 Nov 20;8(12):1677-1685. doi: 10.1039/d3nh00365e.
The increasing prevalence of antibiotic-resistant bacterial infections, particularly methicillin-resistant (MRSA), presents a significant public health concern. Timely detection of MRSA is crucial to enable prompt medical intervention, limit its spread, and reduce antimicrobial resistance. Here, we introduce a miniaturized nano-sieve device featuring a pneumatically-regulated chamber for highly efficient MRSA purification from human plasma samples. By using packed magnetic beads as a filter and leveraging the deformability of the nano-sieve channel, we achieved an on-chip concentration factor of ∼15-fold for MRSA. We integrated this device with recombinase polymerase amplification (RPA) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas detection system, resulting in an on-chip limit of detection (LOD) of approximately 100 CFU mL. This developed approach provides a rapid, precise, and centrifuge-free solution suitable for point-of-care diagnostics, with the potential to significantly improve patient outcomes in resource-limited medical conditions.
耐抗生素细菌感染（尤其是耐甲氧西林金黄色葡萄球菌，MRSA）的日益流行，对公共健康构成了重大威胁。及时检测 MRSA 对于实施及时的医疗干预、限制其传播和减少抗生素耐药性至关重要。在这里，我们引入了一种微型纳米筛装置，该装置具有气动调节腔室，可从人血浆样本中高效纯化 MRSA。我们使用填充的磁性珠作为过滤器，并利用纳米筛通道的可变形性，实现了对 MRSA 的约 15 倍的片上浓缩因子。我们将该装置与重组酶聚合酶扩增（RPA）和簇状规律间隔短回文重复（CRISPR）-Cas 检测系统集成在一起，从而在片上实现了约 100 CFU mL 的检测限（LOD）。这种开发的方法提供了一种快速、精确且无需离心的解决方案，适用于即时护理诊断，有可能显著改善资源有限的医疗条件下的患者预后。
