Li Hui, Liu Hongyi, Liu Yifei, Wang Xuefei, Yu Shiya, Huang Hongwen, Shen Xiangru, Zhang Qi, Hong Ni, Jin Wenfei
School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China.
CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
iScience. 2023 Aug 9;26(9):107588. doi: 10.1016/j.isci.2023.107588. eCollection 2023 Sep 15.
T cell activation is a key event in adaptive immunity. However, the dynamics and influencing factors of T cell activation remain unclear. Here, we analyzed CD4 T cells that were stimulated with anti-CD3/CD28 under several conditions to explore the factors affecting T cell activation. We found a stimulated T subset (HSP T) highly expressing heat shock proteins, which was derived from stimulated naive T. We identified and characterized inert T, a stimulated T cell subset in transitional state from resting T to activated T. Interestingly, resting T responded to stimulation more efficiently than resting T. Furthermore, stimulation of CD4 T in the presence of CD8 T resulted in more effector T and more homogeneous expressions of CD25, supporting that presence of CD8 T reduces the extreme response of T cells, which can be explained by regulation of CD4 T activation through CD8 T-initiated cytokine signaling and FAS/FASLG signaling.
T细胞活化是适应性免疫中的关键事件。然而，T细胞活化的动力学和影响因素仍不清楚。在此，我们分析了在几种条件下用抗CD3/CD28刺激的CD4 T细胞，以探索影响T细胞活化的因素。我们发现了一个高表达热休克蛋白的受刺激T细胞亚群（HSP T），它来源于受刺激的初始T细胞。我们鉴定并表征了惰性T细胞，这是一种处于从静息T细胞到活化T细胞过渡状态的受刺激T细胞亚群。有趣的是，静息T细胞对刺激的反应比静息T细胞更有效。此外，在CD8 T细胞存在的情况下刺激CD4 T细胞会产生更多的效应T细胞以及更均匀的CD25表达，这支持了CD8 T细胞的存在会降低T细胞的极端反应，这可以通过CD8 T细胞启动的细胞因子信号传导和FAS/FASLG信号传导对CD4 T细胞活化的调节来解释。
