Departments of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
J Vet Diagn Invest. 2023 Nov;35(6):664-670. doi: 10.1177/10406387231197440. Epub 2023 Aug 30.
The Platelet Function Analyzer 200 (PFA-200; Siemens) is an in vitro substitute for in vivo bleeding time that is designed to investigate platelet function in a more physiologic manner than traditional aggregometry. The analyzer reports a closure time (CT) as a marker of platelet function, and may also report the calculated platelet function measurement primary hemostasis components, PHC1 and PHC2. These incorporate the measured total volume (TV) of blood aspirated and the initial flow rate (IF). We determined, for the COL/ADP and P2Y cartridges, the median total volume (TV), and RIs for CT, IF, TV, PHC1, and PHC2, and investigated the sensitivity and specificity of those parameters at the determined interpretation thresholds in determination of the clopidogrel effect. Healthy client-owned cats were recruited prospectively to determine RIs for CT, IF, TV, PHC1, and PHC2. Healthy blood-donor cats and cats on clopidogrel therapy were included retrospectively to determine test performance. In 20 healthy cats, RIs for COL/ADP were CT (19.5-87.2 s), IF (199-278 µL/min), TV (199-332 µL), PHC1 (94-106%), and PHC2 (52-148%); and for P2Y, CT (4.2-94.3 s), IF (112-208 µL/min), TV (151-294 µL), PHC1 (35-178%), and PHC2 (90-109%). CVs were calculated for all of these values. Specificity for detection of the clopidogrel effect was calculated from a group of healthy blood donors, and sensitivity for detection of the clopidogrel effect from a group of cats with known clopidogrel effect. Sensitivity and specificity were, for COL/ADP: CT (83.3%, 66.6%), IF (41.4%, 83.3%), TV (83.3%, 100%), PHC1 (100%, 100%) and PHC2 (100%, 83.3%); and for P2Y: CT (100%, 94.4%), IF (30%, 44.4%), TV (100%, 94.4%), PHC1 (100%, 100%), and PHC2 (100%, 97.7%). These PFA-200 values may be beneficial in the determination of platelet function in cats.
血小板功能分析仪 200（PFA-200；西门子）是一种体外替代体内出血时间的方法，旨在以比传统聚集术更生理性的方式研究血小板功能。分析仪报告一个闭合时间（CT）作为血小板功能的标志物，并且还可以报告计算的血小板功能测量主要止血成分，PHC1 和 PHC2。这些包含测量的总体积（TV）和初始流速（IF）。我们确定了 COL/ADP 和 P2Y 试剂盒的中位数总体积（TV）和 CT、IF、TV、PHC1 和 PHC2 的 RI，并在确定的解释阈值处研究了这些参数在确定氯吡格雷作用中的敏感性和特异性。前瞻性招募健康的客户拥有的猫来确定 CT、IF、TV、PHC1 和 PHC2 的 RI。回顾性纳入健康的献血猫和氯吡格雷治疗的猫来确定测试性能。在 20 只健康的猫中，COL/ADP 的 RI 为 CT（19.5-87.2s）、IF（199-278µL/min）、TV（199-332µL）、PHC1（94-106%）和 PHC2（52-148%）；对于 P2Y，RI 为 CT（4.2-94.3s）、IF（112-208µL/min）、TV（151-294µL）、PHC1（35-178%）和 PHC2（90-109%）。计算了所有这些值的 CV。从一组健康献血者中计算了检测氯吡格雷作用的特异性，从一组已知氯吡格雷作用的猫中计算了检测氯吡格雷作用的敏感性。COL/ADP 的敏感性和特异性为 CT（83.3%，66.6%）、IF（41.4%，83.3%）、TV（83.3%，100%）、PHC1（100%，100%）和 PHC2（100%，83.3%）；对于 P2Y：CT（100%，94.4%）、IF（30%，44.4%）、TV（100%，94.4%）、PHC1（100%，100%）和 PHC2（100%，97.7%）。这些 PFA-200 值可能有助于确定猫的血小板功能。
