验证用于分析猫血样本的储存和运输方法，以便在血小板功能分析仪-200 上进行分析，以确定氯吡格雷的效果。
Validation of storage and shipping of feline blood samples for analysis on the Platelet Function Analyzer-200 for determining the effect of clopidogrel.
机构信息
Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
出版信息
Vet Clin Pathol. 2023 Dec;52(4):588-595. doi: 10.1111/vcp.13265. Epub 2023 Jul 24.
BACKGROUND
The Platelet function analyzer-200 (PFA-200) can determine the effect of clopidogrel in cats, but analysis traditionally must be performed at point-of-care (POC). The ability to ship samples of blood to a laboratory would allow widespread access.
OBJECTIVES
We aimed to validate the shipping of blood samples for PFA-200 analysis in cats to determine the effect of clopidogrel.
METHODS
Twenty healthy cats and 10 cats receiving clopidogrel were recruited. Blood was collected from cats and aliquoted into two samples, one was analyzed at POC within 2 hours using the PFA-200, and the other was packaged and transported to a location 4 km away, stored, and transported back to the lab for analysis the following day.
RESULTS
Median closure times (CTs) with the collagen/adenosine diphosphate (COL/ADP) cartridge in healthy cats were 51.5 seconds (POC) and 78.8 seconds (shipped), which were significantly different (P < 0.001), and for cats on clopidogrel, median CTs were 147.5 seconds (POC) and 190 seconds (shipped), which were not significantly different (P = 0.131). Median CTs with the P2Y cartridge in healthy cats were 50.5 seconds (POC) and 64.9 seconds (shipped), which were significantly different (P = 0.03), and in cats receiving clopidogrel, median CTs were 300 seconds (POC) and 300 seconds (shipped) which were not significantly different (P = 1.000). Reference intervals for CTs differed for COL/ADP at POC (19.8-89.7 seconds) and shipped (50.9-161.6 seconds) and for P2Y at POC (35.5-118.8 seconds) and shipped (35.1-108.9 seconds). Receiver operating characteristics showed similar areas under the curve (AUCROCs) regarding the effect of clopidogrel for COL/ADP at POC (0.994 seconds) and shipped (0.932) and for P2Y at POC (0.904 seconds) and shipped (0.975 seconds). When classifying for the presence of clopidogrel effects, Cohen's Kappa was 0.62 for COL/ADP and 1.00 for P2Y.
CONCLUSIONS
Shipping blood samples for PFA analysis are feasible with similar performance to POC analyses for determining the effect of clopidogrel in cats.
背景
血小板功能分析仪-200（PFA-200）可用于检测猫体内氯吡格雷的作用，但传统分析必须在床边进行。如果能够将血样运送到实验室，将会有更多的猫受益。
目的
本研究旨在验证通过运输猫的血样进行 PFA-200 分析以确定氯吡格雷作用的可行性。
方法
共招募了 20 只健康猫和 10 只接受氯吡格雷治疗的猫。采集猫的血液并分成两份样本，一份在 2 小时内使用 PFA-200 在床边进行分析，另一份包装并运输到 4 公里外的地方储存，第二天再运回到实验室进行分析。
结果
健康猫的胶原/腺苷二磷酸（COL/ADP）检测通道的中位闭合时间（CT）在床边为 51.5 秒（POC）和 78.8 秒（运输），差异有统计学意义（P<0.001），而接受氯吡格雷治疗的猫的中位 CT 为 147.5 秒（POC）和 190 秒（运输），差异无统计学意义（P=0.131）。健康猫的 P2Y 检测通道的中位 CT 在床边为 50.5 秒（POC）和 64.9 秒（运输），差异有统计学意义（P=0.03），而接受氯吡格雷治疗的猫的中位 CT 为 300 秒（POC）和 300 秒（运输），差异无统计学意义（P=1.000）。COL/ADP 在床边的参考区间（19.8-89.7 秒）和运输后的参考区间（50.9-161.6 秒）不同，P2Y 在床边的参考区间（35.5-118.8 秒）和运输后的参考区间（35.1-108.9 秒）也不同。接受者操作特征曲线（ROC）显示，COL/ADP 在床边（0.994 秒）和运输后（0.932）以及 P2Y 在床边（0.904 秒）和运输后（0.975 秒）的氯吡格雷效应的 AUCROC 相似。当对氯吡格雷的作用进行分类时，COL/ADP 的 Cohen's Kappa 为 0.62，而 P2Y 的 Cohen's Kappa 为 1.00。
结论
通过运输血样进行 PFA 分析是可行的，与床边分析相比，这种方法在确定猫体内氯吡格雷的作用方面具有相似的性能。
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