Study Program of Chemistry, Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia, Bandung, Indonesia.
Laboratory of Biopharmaceutics, Department of Pharmacology, Faculty of Pharmacy, Takasaki University of Health and Welfare, Takasaki, Japan.
Bioengineered. 2023 Dec;14(1):2252226. doi: 10.1080/21655979.2023.2252226.
Phycocyanin, produced by has been reported as an anti-inflammatory, anti-hyperalgesia, antioxidant, anti-tumor, and anti-cancer agent. However, the ingestion of phycocyanin in the body is often hindered by its instability against gastric pH conditions. The nano-drug delivery system has developed as a promising platform for efficient drug delivery and improvement as well as drug efficacy. Bacterial cellulose nanocrystal (BCNC) has it superiority as DDS due to its inherent properties such as nanoscale dimension, large surface area, - biocompatibility, and non-toxic. To improve its mechanical properties, BCNC was crosslinked with glutaraldehyde and was analyzed as a potential candidate for DDS. The Fourier transform infrared analysis of the BCNC suggested that hydrolysis did not alter the chemical composition. The index of crystallinity of the BCNC was 18.31% higher than that of the original BC, suggesting that crystalline BC has been successfully isolated. The BCNC particle also showed a needle-like morphology which is 25 ± 10 nm in diameter and a mean length of 626 ± 172 nm. Crosslinked BCNC also had larger pores than the original BCNC along with higher thermal stability. Optimum phycocyanin adsorption on crosslinked BCNC reached 65.3% in 3 h. The release study shows that the crosslinked BCNC can protect the phycocyanin retardation by gastric fluid until phycocyanin reaches the targeted sites. This study provides an alternative potential DDS derived from natural bioresources with less expenses and better properties to promote the application of BCNC as functional nanomaterials in biomedical science.
藻蓝蛋白由 产生，已被报道具有抗炎、抗痛觉过敏、抗氧化、抗肿瘤和抗癌作用。然而，藻蓝蛋白在体内的摄入常常受到其对胃 pH 值条件不稳定的阻碍。纳米药物递送系统已作为一种有前途的高效药物递送和改善以及药物功效的平台而发展。由于其固有特性，如纳米尺寸、大表面积、生物相容性和无毒，细菌纤维素纳米晶体 (BCNC) 作为 DDS 具有优势。为了提高其机械性能，BCNC 用戊二醛交联，并被分析为 DDS 的潜在候选物。BCNC 的傅里叶变换红外分析表明，水解没有改变其化学成分。BCNC 的结晶度指数比原始 BC 高 18.31%，表明已成功分离出结晶 BC。BCNC 颗粒还呈现出针状形态，直径为 25±10nm，平均长度为 626±172nm。交联 BCNC 也具有比原始 BCNC 更大的孔和更高的热稳定性。藻蓝蛋白在交联 BCNC 上的最佳吸附在 3 小时内达到 65.3%。释放研究表明，交联 BCNC 可以保护藻蓝蛋白免受胃液的延迟释放，直到藻蓝蛋白到达靶向部位。这项研究提供了一种替代的潜在的 DDS，来源于天然生物资源，费用更低，性能更好，以促进 BCNC 作为功能纳米材料在生物医学科学中的应用。
