Yang Yi, Liu Yanyun, Xia Yuqing, Cheng Jianxin, Liu Pengfei
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University) Ministry of Education, 116023, China.
Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University) Ministry of Education, 116023, China; School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2023 Dec;48:101124. doi: 10.1016/j.cbd.2023.101124. Epub 2023 Aug 22.
In this study, we identified the differentially expressed proteins in gills stimulated by infected ciliates and analyzed the immune mechanisms of T. rubripes infected with the ciliate Cryptocaryon irritans. Through liquid chromatography analysis, a total of 144 proteins were identified with significant differences, of which 58 were upregulated and 86 were downregulated. Among phosphorylated proteins, we identified a total of 167 significantly different phosphorylated proteins, of which 44 were upregulated, 123 were downregulated, 60 were upregulated, and 208 were downregulated. We analyzed the data of proteomics and Phosphorylated proteome quantification protein omics to finally identify three phosphorylated proteins (RPS27, eNOS and CaM) and two phosphorylated protein kinases(CaMKII and MAPK1) as potential biomarkers for T. rubripes immune responses. We finally identified three phosphorylated proteins (RPS27, eNOS and CaM) and two phosphorylated protein kinases (CaMKII and MAPK1) as potential biomarkers of immune response of T. rubripes. Our research findings provide new insights into the immune mechanism of T. rubripes, which may serve as an effective indicator of C. irritans infection in T. rubripes.
在本研究中，我们鉴定了受感染纤毛虫刺激的鳃中差异表达的蛋白质，并分析了感染刺激隐核虫的红鳍东方鲀的免疫机制。通过液相色谱分析，共鉴定出144种差异显著的蛋白质，其中58种上调，86种下调。在磷酸化蛋白质中，我们共鉴定出167种差异显著的磷酸化蛋白质，其中44种上调，123种下调，60种上调，208种下调。我们分析了蛋白质组学和磷酸化蛋白质组定量蛋白质组学的数据，最终鉴定出三种磷酸化蛋白质（RPS27、eNOS和CaM）和两种磷酸化蛋白激酶（CaMKII和MAPK1）作为红鳍东方鲀免疫反应的潜在生物标志物。我们最终鉴定出三种磷酸化蛋白质（RPS27、eNOS和CaM）和两种磷酸化蛋白激酶（CaMKII和MAPK1）作为红鳍东方鲀免疫反应的潜在生物标志物。我们的研究结果为红鳍东方鲀的免疫机制提供了新的见解，这可能作为红鳍东方鲀感染刺激隐核虫的有效指标。
