College of Marine Technology and Environment, Dalian Ocean University, Dalian, 116023, China; Key Laboratory of Environment Controlled Aquaculture (KLECA), Ministry of Education, 52 Heishijiao Street, Dalian, 116023, China.
School of Marine Sciences, Ningbo University, Ningbo, 315211, China.
Fish Shellfish Immunol. 2020 Sep;104:213-221. doi: 10.1016/j.fsi.2020.06.008. Epub 2020 Jun 10.
Cryptocaryon irritans can cause cryptocaryonosis (white spot disease) in marine fish but the pathogenesis of the disease is unclear. In this work, we used high-throughput proteomics to identify differentially expressed proteins in the serum of Takifugu rubripes challenged with C. irritans. By using quantitative proteomic assays combined with Tandem Mass Tag-labeled quantitative proteomic analysis, we identified a total of 2088 differentially abundant proteins (1706 proteins were quantified, p < 0.05, fold-change threshold ≥ 2), including 21 up-regulated and 44 down-regulated. Combined with STRING-based functional analysis, we ultimately obtained eight proteins including glucokinase-like, integrin beta-1-like isoform X2, H4, H2A.V, histone H1-like, histone H2AX-like, histone H2B 1/2-like and myosin-9 isoform X1, which could be considered as potential biomarkers for T. rubripes immune responses. Eight proteins that were selected to validate significant differentially expressed genes at the proteomic level were consistent with qPCR at the transcriptomic level. The proteins identified in our work may serve as candidates for elucidating the molecular mechanism of cryptocaryonosis in T. rubripes. Our collective findings could provide new insights into searching for disease-specific targets and biomarkers, which may be effective indicators of C. irritans infection in T. rubripes.
刺激隐核虫可引起海水鱼类的刺激隐核虫病（白点病），但其发病机制尚不清楚。在这项工作中，我们使用高通量蛋白质组学鉴定了受到刺激隐核虫攻击的红鳍东方鲀血清中的差异表达蛋白。通过使用定量蛋白质组学测定结合串联质量标签标记定量蛋白质组学分析，我们总共鉴定了 2088 种差异丰度蛋白（1706 种蛋白被定量，p<0.05，倍数变化阈值≥2），包括 21 种上调蛋白和 44 种下调蛋白。结合基于 STRING 的功能分析，我们最终获得了 8 种蛋白，包括葡萄糖激酶样蛋白、整合素β-1 样异构体 X2、H4、H2A.V、组蛋白 H1 样蛋白、组蛋白 H2AX 样蛋白、组蛋白 H2B1/2 样蛋白和肌球蛋白-9 异构体 X1，它们可被视为红鳍东方鲀免疫反应的潜在生物标志物。在蛋白质组学水平上选择验证差异表达基因的 8 种蛋白与转录组学水平上的 qPCR 一致。我们鉴定的蛋白可能有助于阐明红鳍东方鲀刺激隐核虫病的分子机制。我们的研究结果可为寻找疾病特异性靶标和生物标志物提供新的思路，这些标志物可能是刺激隐核虫感染红鳍东方鲀的有效指标。
