College of Pharmaceutical Science & Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014, China.
College of Pharmaceutical Science & Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou 310014, China.
Fitoterapia. 2023 Oct;170:105662. doi: 10.1016/j.fitote.2023.105662. Epub 2023 Aug 28.
Two new terrein derivatives asperterreinones A-B (1-2), one new octahydrocoumarin derivative (±)-asperterreinin A (6), along with seventeen known compounds, were isolated from Aspergillus terreus F6-3, a marine fungus associated with Johnius belengerii. The structures of 1, 2, and 6 were established on the basis of 1D and 2D NMR, mass spectroscopy, comparative electronic circular dichroism (ECD) spectra analysis, density functional theory calculation of C NMR, and DP4+ probability analysis. Among all the isolates, eurylene (7), a constituent of the Malaysian medicinal plant Eurycoma longifolia, was obtained from a microbial source for first time. In the in vitro bioassay, 11 and 13 showed potent inhibitory activity against the Escherichia coli β-glucuronidase (EcGUS) with IC values of 27.75 ± 0.73 and 17.73 ± 0.81 μM, respectively. It was the first time that questinol (11) and (±)-aspertertone B (13) were reported as potent EcGUS inhibitors.
从海洋真菌与黄鳍鲷相关的曲霉菌 F6-3 中分离得到两个新的土青霉烯衍生物 Asperterreinones A-B（1-2），一个新的八氢香豆素衍生物（±）- Asperterreinin A（6），以及十七个已知化合物。基于 1D 和 2D NMR、质谱、比较电子圆二色性（ECD）光谱分析、C NMR 的密度泛函理论计算和 DP4+概率分析，确定了 1、2 和 6 的结构。在所有分离物中，eurylene（7）是从微生物来源获得的，首次从马来西亚药用植物长叶羽扇豆中获得。在体外生物测定中，化合物 11 和 13 对大肠杆菌β-葡萄糖醛酸酶（EcGUS）显示出强烈的抑制活性，IC 值分别为 27.75±0.73 和 17.73±0.81 μM。这是首次报道 questinol（11）和（±）- Aspertertone B（13）是有效的 EcGUS 抑制剂。
