血清 IgE 介导的食物过敏儿童的 16S rRNA 基因测序微生物组比较。

Comparison of 16S rRNA gene sequencing microbiota among children with serological IgE-mediated food hypersensitivity.

机构信息

Division of Gastroenterology, Department of Pediatrics, Chang Gung Memorial Hospital, Taoyuan, Taiwan.

Chang Gung University College of Medicine, Taoyuan, Taiwan.

出版信息

Pediatr Res. 2024 Jan;95(1):241-250. doi: 10.1038/s41390-023-02735-7. Epub 2023 Aug 30.

DOI:10.1038/s41390-023-02735-7

PMID:37648747

Abstract

BACKGROUND

We hypothesized that specific food hypersensitivity (FH) in children is linked to specific gut microbiota. The aim of our study was to quantify and evaluate differences in gut microbial composition among children with different IgE-mediated FH.

METHODS

Children (n = 81) aged 18 to 36 months were enrolled, fecal samples of 57 children with FH and 24 healthy children were evaluated using next-generation sequencing. Individual microbial diversity and composition were analyzed via targeting the 16 S rRNA gene hypervariable V3-V5 regions.

RESULTS

Children with IgE-mediated FH (in milk, egg white, soy) had significantly lower gut microbiota diversity and richness than healthy children. Children with IgE-mediated FH exhibited relatively high abundances of Firmicutes and relative underrepresentation of the phylum Bacteroidetes. We observed significant increases in relative abundances of Ruminococcaceae, Clostridiaceae, and Erysipelotrichaceae (p < 0.01, compared to control) in children with milk hypersensitivity and of Clostridiaceae and Erysipelotrichaceae (p < 0.01) in children with peanut hypersensitivity. We also found significant increases in the numbers of Clostridiaceae, Lachnospiraceae and Pasteurellaceae (p < 0.01) in children with egg white hypersensitivity.

CONCLUSIONS

These findings identify early evidence of different gut microbiota development/ differentiation in children with food hypersensitivity. Specific food hypersensitivities may be associated with compositional changes in intestinal microbiota.

IMPACT

These findings identify early evidence of different gut microbiota development/differentiation in children with food hypersensitivity. We built a gut microbial profile that could identify toddlers at risk for food hypersensitivity. Children with enriched Firmicutes (phylum) with partial different families may be associated with food hypersensitivity. Enriched family Clostridiaceae, Ruminococcaceae, Lachnospiraceae, or Erysipelotrichaceae in gut microbiota may be associated with specific food hypersensitivities (such as milk, egg white, peanut) in children.

摘要

背景

我们假设儿童特定食物过敏（FH）与特定的肠道微生物群有关。我们研究的目的是量化和评估不同 IgE 介导的 FH 儿童肠道微生物组成的差异。

方法

招募了 18 至 36 个月大的儿童，对 57 名 FH 儿童和 24 名健康儿童的粪便样本进行了下一代测序评估。通过靶向 16S rRNA 基因高变区 V3-V5 区域分析个体微生物多样性和组成。

结果

与健康儿童相比，IgE 介导的 FH（牛奶、蛋清、大豆）儿童的肠道微生物多样性和丰富度显著降低。IgE 介导的 FH 儿童表现出相对较高的厚壁菌门丰度和较低的拟杆菌门相对丰度。我们观察到，牛奶过敏儿童中瘤胃球菌科、梭菌科和埃希氏菌科的相对丰度显著增加（与对照组相比，p < 0.01），花生过敏儿童中梭菌科和埃希氏菌科的相对丰度显著增加（p < 0.01）。我们还发现，蛋清过敏儿童中梭菌科、毛螺菌科和巴斯德氏菌科的数量显著增加（p < 0.01）。

结论

这些发现为食物过敏儿童肠道微生物群的不同发育/分化提供了早期证据。特定的食物过敏可能与肠道微生物群的组成变化有关。

影响

这些发现为食物过敏儿童肠道微生物群的不同发育/分化提供了早期证据。我们构建了一个肠道微生物特征，可以识别出有食物过敏风险的幼儿。富含厚壁菌门（门）的儿童，其部分不同的家族可能与食物过敏有关。肠道微生物群中富集的梭菌科、瘤胃球菌科、毛螺菌科或埃希氏菌科可能与儿童特定的食物过敏（如牛奶、蛋清、花生）有关。