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微小巴贝斯虫的一种保守蛋白引发针对巴贝斯虫和疟原虫感染的部分保护。

A conserved protein of Babesia microti elicits partial protection against Babesia and Plasmodium infection.

机构信息

School of Biology and Basic Medical Sciences, Soochow University, No.199 Renai Road, Suzhou, 215123, People's Republic of China.

Institutes of Biology and Medical Sciences, Jiangsu Key Laboratory of Infection and Immunity, Soochow University, No.199 Renai Road, Suzhou, 215123, People's Republic of China.

出版信息

Parasit Vectors. 2023 Aug 30;16(1):306. doi: 10.1186/s13071-023-05825-x.

DOI:10.1186/s13071-023-05825-x
PMID:37649042
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10469411/
Abstract

BACKGROUND

The protozoan parasite Babesia microti that causes the zoonotic disease babesiosis resides in the erythrocytes of its mammalian host during its life-cycle. No effective vaccines are currently available to prevent Babesia microti infections.

METHODS

We previously identified a highly seroactive antigen, named Bm8, as a B. microti conserved erythrocyte membrane-associated antigen, by high-throughput protein chip screening. Bioinformatic and phylogenetic analysis showed that this membrane-associated protein is conserved among apicomplexan hemoprotozoa, such as members of genera Babesia, Plasmodium and Theileria. We obtained the recombinant protein Bm8 (rBm8) by prokaryotic expression and purification.

RESULTS

Immunofluorescence assays confirmed that Bm8 and its Plasmodium homolog were principally localized in the cytoplasm of the parasite. rBm8 protein was specifically recognized by the sera of mice infected with B. microti or P. berghei. Also, mice immunized with Bm8 polypeptide had a decreased parasite burden after B. microti or P. berghei infection.

CONCLUSIONS

Passive immunization with Bm8 antisera could protect mice against B. microti or P. berghei infection to a certain extent. These results lead us to hypothesize that the B. microti conserved erythrocyte membrane-associated protein Bm8 could serve as a novel broad-spectrum parasite vaccine candidate since it elicits a protective immune response against Babesiosis and Plasmodium infection.

摘要

背景

引起动物源性疾病巴贝斯虫病的原生动物寄生虫巴贝斯微孢子虫在其生命周期中存在于哺乳动物宿主的红细胞中。目前尚无有效的疫苗可预防巴贝斯微孢子虫感染。

方法

我们之前通过高通量蛋白芯片筛选鉴定了一种高度血清反应性抗原，命名为 Bm8，它是一种巴贝斯微孢子虫保守的红细胞膜相关抗原。生物信息学和系统发育分析表明，这种膜相关蛋白在顶复门血原虫中是保守的，如巴贝斯虫属、疟原虫属和泰勒虫属的成员。我们通过原核表达和纯化获得了重组蛋白 Bm8（rBm8）。

结果

免疫荧光分析证实 Bm8 及其疟原虫同源物主要定位于寄生虫的细胞质中。rBm8 蛋白被感染巴贝斯微孢子虫或感染疟原虫的小鼠血清特异性识别。此外，用 Bm8 多肽免疫的小鼠在感染巴贝斯微孢子虫或疟原虫后寄生虫负荷减少。

结论

用 Bm8 抗血清进行被动免疫可在一定程度上保护小鼠免受巴贝斯微孢子虫或疟原虫感染。这些结果使我们假设，巴贝斯微孢子虫保守的红细胞膜相关蛋白 Bm8 可以作为一种新型广谱寄生虫疫苗候选物，因为它可以针对巴贝斯虫病和疟原虫感染产生保护性免疫反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aacd/10469411/a98378ef5dd9/13071_2023_5825_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aacd/10469411/b007f3563be5/13071_2023_5825_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aacd/10469411/e64cf7235efe/13071_2023_5825_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aacd/10469411/26cc351db380/13071_2023_5825_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aacd/10469411/f4add271daa3/13071_2023_5825_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aacd/10469411/22d745deaed4/13071_2023_5825_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aacd/10469411/a98378ef5dd9/13071_2023_5825_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aacd/10469411/b007f3563be5/13071_2023_5825_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aacd/10469411/e64cf7235efe/13071_2023_5825_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aacd/10469411/26cc351db380/13071_2023_5825_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aacd/10469411/f4add271daa3/13071_2023_5825_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aacd/10469411/22d745deaed4/13071_2023_5825_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aacd/10469411/a98378ef5dd9/13071_2023_5825_Fig6_HTML.jpg

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