葡萄糖可改善百草枯对大鼠肺切片中烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的消耗。

Glucose ameliorates the depletion of NADPH by paraquat in rat lung slices.

作者信息

Sullivan T M, Montgomery M R

出版信息

Toxicology. 1986 Oct;41(2):145-52. doi: 10.1016/0300-483x(86)90195-2.

Abstract

Paraquat-stimulated NADPH depletion in rat lung slices is responsive to exogenous glucose concentration. Lung slices incubated with 11 mM glucose and 10(-4) M paraquat had a 40% lower NADPH/NADP+ ratio than did control lung slices. Incubation with no added glucose and 10(-5) M paraquat caused a 41% decrease in NADPH/NADP+. With paraquat at 10(-5) M, glucose at 1.1, 5.5, 11, or 22 mM increased NADPH/NADP+ ratios in a concentration-dependent manner until, at 22 mM glucose, the effect of paraquat was prevented. The sum of NADP+ plus NADPH was only 60% of control with 10(-5) M paraquat and no glucose. However, with any concentration of glucose from 1.1 to 22 mM, the total was 92% of control. The results indicate that glucose may be beneficial in preventing paraquat-mediated NADPH depletion in rat lung slices.

摘要

百草枯刺激大鼠肺切片中NADPH的消耗对外源葡萄糖浓度有反应。用11 mM葡萄糖和10⁻⁴ M百草枯孵育的肺切片，其NADPH/NADP⁺比值比对照肺切片低40%。在不添加葡萄糖和10⁻⁵ M百草枯的情况下孵育，导致NADPH/NADP⁺降低41%。当百草枯浓度为10⁻⁵ M时，1.1、5.5、11或22 mM的葡萄糖以浓度依赖的方式增加NADPH/NADP⁺比值，直到在22 mM葡萄糖时，百草枯的作用被阻止。在10⁻⁵ M百草枯且无葡萄糖的情况下，NADP⁺加NADPH的总和仅为对照的60%。然而，在1.1至22 mM的任何葡萄糖浓度下，总和为对照的92%。结果表明，葡萄糖可能有助于防止百草枯介导的大鼠肺切片中NADPH的消耗。