ICAR-National Rice Research Institute (NRRI), Cuttack, Odisha, India.
ICAR-NRRI, Regional Research Station (CRURRS), Hazaribagh, Jharkhand.
PLoS One. 2020 Jul 16;15(7):e0227785. doi: 10.1371/journal.pone.0227785. eCollection 2020.
A panel of 60 genotypes comprising New Plant Types (NPTs) along with indica, tropical and temperate japonica genotypes was phenotypically evaluated for four seasons in irrigated situation for grain yield per se and component traits. Twenty NPT genotypes were found promising with an average grain yield varying from 5.45 to 8.8 t/ha. A total of 85 SSR markers were used in the study to identify QTLs associated with grain yield per se and related traits. Sixty-six (77.65%) markers were found to be polymorphic. The PIC values varied from 0.516 to 0.92 with an average of 0.704. A moderate level of genetic diversity (0.39) was detected among genotypes. Variation to the tune of 8% within genotypes, 68% among the genotypes within the population and 24% among the populations were observed (AMOVA). This information may help in identification of potential parents for development of transgressive segregants with very high yield. The association analysis using GLM and MLM models led to the identification of 30 and 10 SSR markers associated with 70 and 16 QTLs, respectively. Thirty novel QTLs linked with 16 SSRs were identified to be associated with eleven traits, namely tiller number (qTL-6.1, qTL-11.1, qTL-4.1), panicle length (qPL-1.1, qPL-5.1, qPL-7.1, qPL-8.1), flag leaf length (qFLL-8.1, qFLL-9.1), flag leaf width (qFLW-6.2, qFLW-5.1, qFLW-8.1, qFLW-7.1), total no. of grains (qTG-2.2, qTG-a7.1), thousand-grain weight (qTGW-a1.1, qTGW-a9.2, qTGW-5.1, qTGW-8.1), fertile grains (qFG-7.1), seed length-breadth ratio (qSlb-3.1), plant height (qPHT-6.1, qPHT-9.1), days to 50% flowering (qFD-1.1) and grain yield per se (qYLD-5.1, qYLD-6.1a, qYLD-11.1).Some of the SSRs were co-localized with more than two traits. The highest co-localization was identified with RM5709 linked to nine traits, followed by RM297 with five traits. Similarly, RM5575, RM204, RM168, RM112, RM26499 and RM22899 were also recorded to be co-localized with more than one trait and could be rated as important for marker-assisted backcross breeding programs, for pyramiding of these QTLs for important yield traits, to produce new-generation rice for prospective increment in yield potentiality and breaking yield ceiling.
60 个基因型的小组包括新植物类型（NPT）以及籼稻、热带和温带粳稻基因型，在灌溉条件下进行了四个季节的表型评估，以评估其自身的籽粒产量和组成性状。发现 20 个 NPT 基因型有希望，平均籽粒产量在 5.45 到 8.8 吨/公顷之间。总共使用了 85 个 SSR 标记来鉴定与自身籽粒产量和相关性状相关的 QTL。66（77.65%）个标记被发现是多态的。PIC 值从 0.516 到 0.92，平均为 0.704。基因型之间检测到中等水平的遗传多样性（0.39）。在基因型内观察到 8%的变异、在群体内的 68%的基因型和在群体间的 24%的变异（AMOVA）。这些信息可能有助于鉴定潜在的亲本，以开发具有超高产量的转基因分离群体。使用 GLM 和 MLM 模型进行的关联分析鉴定了 30 个和 10 个与 70 个和 16 个 QTL 分别相关的 SSR 标记。鉴定出 30 个与 16 个 SSR 相关的新 QTL 与 11 个性状相关，即分蘖数（qTL-6.1、qTL-11.1、qTL-4.1）、穗长（qPL-1.1、qPL-5.1、qPL-7.1、qPL-8.1）、旗叶长（qFLL-8.1、qFLL-9.1）、旗叶宽（qFLW-6.2、qFLW-5.1、qFLW-8.1、qFLW-7.1）、总粒数（qTG-2.2、qTG-a7.1）、千粒重（qTGW-a1.1、qTGW-a9.2、qTGW-5.1、qTGW-8.1）、结实粒数（qFG-7.1）、粒长-粒宽比（qSlb-3.1）、株高（qPHT-6.1、qPHT-9.1）、抽穗期至 50%（qFD-1.1）和自身籽粒产量（qYLD-5.1、qYLD-6.1a、qYLD-11.1）。一些 SSR 与两个以上性状共定位。与 9 个性状共定位的 RM5709 最多，其次是 RM297 与 5 个性状共定位。同样，RM5575、RM204、RM168、RM112、RM26499 和 RM22899 也被记录为与一个以上性状共定位，可作为标记辅助回交育种计划的重要标记，用于聚合这些重要产量性状的 QTL，以产生新一代水稻，提高未来的产量潜力和打破产量上限。
