Cheng Lishu, Wei Shuang, Liu Kuocheng, Zhao Xu, Zhang Jun, Zhao Yan
College of Life Science and Agroforestry, Qiqihar University, Wenhua Street, No. 42, Qiqihar, 161006 Heilongjiang China.
Jilin Province Institute of Product Quality Supervision and Inspection, Changchun, 130022 China.
Physiol Mol Biol Plants. 2023 Jul;29(7):947-957. doi: 10.1007/s12298-023-01342-4. Epub 2023 Aug 18.
The expression of the soybean Bowman-Birk proteinase isoinhibitor DII () gene and the inducible activity of its promoter were studied under salt, drought, low temperature, and abscisic acid (ABA) exposure conditions. The gene was induced by salt, drought, low temperature, and ABA, and the relative expression levels were 103.09-, 107.01-, 17.25- and 27.24-fold, respectively, compared with the untreated control. The putative promoter, designated BP1 (- 1255 to + 872 bp), located 5'-upstream of the gene was cloned. The expression of the gene in pCAM-BP1 transgenic tobacco plants was highest at 5 h after treatment with salt, drought, low temperature and ABA, especially under salt and drought. Using histochemical staining and fluorescence analysis of GUS, BP1 activity under salt and drought conditions after 5 h was 1.03 and 1.07-fold, respectively, compared with that of the CaMV35S promoter. Based on a 5' deletion analysis, the segment (+ 41 to + 474 bp) was the basal region that responded to salt and drought, whereas the segment (- 820 to + 41 bp) was the area that responded to increased salt and drought activity. The BP2 (- 820 to + 872) activities were 0.98- and 1.02-fold compared with that of BP1 under salt and drought conditions and was 435 bp shorter than BP1. The salt- and drought-inducible activities of the BP2 promoter in the roots, stems, and leaves of transgenic tobacco plants were stable. Taken together, BP2 is more suitable than the BP1 promoter for the study and molecular breeding of stress-resistant soybean plants.
研究了大豆Bowman-Birk蛋白酶抑制因子DII（）基因在盐、干旱、低温和脱落酸（ABA）处理条件下的表达及其启动子的诱导活性。该基因受盐、干旱、低温和ABA诱导，与未处理对照相比，相对表达水平分别为103.09倍、107.01倍、17.25倍和27.24倍。克隆了位于该基因5'上游的推定启动子，命名为BP1（-1255至+872 bp）。pCAM-BP1转基因烟草植株中该基因在盐、干旱、低温和ABA处理5 h后表达最高，尤其是在盐和干旱条件下。通过GUS组织化学染色和荧光分析，盐和干旱处理5 h后BP1活性分别是CaMV35S启动子的1.03倍和1.07倍。基于5'缺失分析，（+41至+474 bp）片段是响应盐和干旱的基础区域，而（-820至+41 bp）片段是响应盐和干旱活性增强的区域。在盐和干旱条件下，BP2（-820至+872）活性分别是BP1的0.98倍和1.02倍，且比BP1短435 bp。BP2启动子在转基因烟草植株根、茎和叶中的盐和干旱诱导活性稳定。综上所述，在抗逆大豆植株的研究和分子育种中，BP2比BP1启动子更合适。
