血小板黏附和吞噬作用。一种检测血小板抗体的方法。
Platelet adherence and phagocytosis. A method for the detection of platelet antibodies.
作者信息
MacPherson B R, Cornish N
出版信息
Transfusion. 1986 Sep-Oct;26(5):467-70. doi: 10.1046/j.1537-2995.1986.26587020128.x.
An assay was developed to identify platelet antibodies based on the adherence and phagocytosis of sensitized platelets by blood monocytes. Platelets obtained from normal HLA-typed donors were labeled with carboxyfluorescein diacetate (COFDA), fixed in paraformaldehyde, and sensitized with antibody. T cell-depleted mononuclear cells (MNCs) were mixed with antibody-sensitized platelets. Following an incubation phase, monocytes were isolated by adherence to glass tissue culture slides. Phagocytosis and adherence were evaluated by fluorescence microscopy. Thirteen of 18 donor-specific HLA antibodies and four of six sera from patients with chronic immune thrombocytopenic purpura enhanced platelet uptake. This assay was useful in detecting allo- and autoantibodies directed against platelets and may be valuable in investigating the pathophysiology of macrophage-mediated platelet destruction.
基于血液单核细胞对致敏血小板的黏附和吞噬作用，开发了一种鉴定血小板抗体的检测方法。从正常HLA分型供体获得的血小板用羧基荧光素二乙酸酯（COFDA）标记，用多聚甲醛固定，并用抗体致敏。去除T细胞的单核细胞（MNCs）与抗体致敏的血小板混合。孵育阶段后，通过黏附到玻璃组织培养载玻片上分离单核细胞。通过荧光显微镜评估吞噬作用和黏附情况。18种供体特异性HLA抗体中的13种以及来自慢性免疫性血小板减少性紫癜患者的6份血清中的4份增强了血小板摄取。该检测方法有助于检测针对血小板的同种抗体和自身抗体，在研究巨噬细胞介导的血小板破坏的病理生理学方面可能具有重要价值。
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