尼泊尔急性结膜炎相关的异源病原体特征。
Heterogenous pathogen profile associated with acute conjunctivitis in Nepal.
机构信息
B.P. Koira la Lions Center for Ophthalmic Studies, Institute of Medicine, Tribhuvan University, Kathmandu 44600, Nepal.
F. I. Proctor Foundation, University of California San Francisco, San Francisco, California 94158, USA.
出版信息
Int Health. 2024 Jul 2;16(4):468-470. doi: 10.1093/inthealth/ihad079.
BACKGROUND
Infectious conjunctivitis is common in Nepal.
MATERIALS AND METHODS
This prospective study recruited 60 patients with presumed acute infectious conjunctivitis from the B.P. Koirala Lions Center for Ophthalmic Studies in Kathmandu, Nepal. Swabs from the conjunctiva and anterior nares were processed for metagenomic RNA deep sequencing (RNA-seq).
RESULTS
Pathogens were identified in 55% of cases. RNA viruses were the most common pathogen class identified. Severe acute respiratory syndrome coronavirus 2 was the most common RNA virus identified.
CONCLUSIONS
Acute infectious conjunctivitis varies by location. Contrary to expectations, RNA viruses predominated. Repeat surveillance may be useful and RNA-seq allows for detection of the unexpected pathogen including RNA viruses.
背景
传染性结膜炎在尼泊尔很常见。
材料与方法
本前瞻性研究招募了 60 名来自尼泊尔加德满都 B.P. Koirala 狮子眼科研究中心的疑似急性传染性结膜炎患者。从结膜和前鼻拭子中提取样品，进行宏基因组 RNA 深度测序（RNA-seq）。
结果
55%的病例中确定了病原体。RNA 病毒是最常见的病原体类别。鉴定出的最常见 RNA 病毒是严重急性呼吸综合征冠状病毒 2。
结论
急性传染性结膜炎的发病情况因地理位置而异。与预期相反，RNA 病毒占主导地位。重复监测可能有用，RNA-seq 可检测到意想不到的病原体，包括 RNA 病毒。
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