基于串行控制的多重数字微流控及其在葡萄糖感测中的应用。

Multiplex digital microfluidics using serial controls and its applications in glucose sensing.

机构信息

Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen 518060, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen 518060, China.

出版信息

SLAS Technol. 2024 Apr;29(2):100105. doi: 10.1016/j.slast.2023.08.005. Epub 2023 Aug 29.

DOI:10.1016/j.slast.2023.08.005

PMID:37652174

Abstract

Digital microfluidics (DMF) has found great applications in vitro diagnostics (IVD). Compared to the microfabrication-based DMF, printed circuit board (PCB)-based DMF is more economical and compatible with existing IVD instruments. Despite that, current PCB-based DMF is oftentimes limited by the available droplets that can be controlled simultaneously, compromising their throughput and applications as point-of-care tools. In this work, a platform that simultaneously controls multiple PCB-based DMF plates was constructed. The software and hardware were first developed, followed by the reliability tests. Colorimetric analysis of glucose was applied to the PCB-based DMF, demonstrating the capability of this platform. With the high throughput enabled by simultaneous operations of multiple plates, this PCB-based DMF can potentially allow point-of-care testing with low cost for resource-limited settings.

摘要

数字微流控（DMF）在体外诊断（IVD）中有着广泛的应用。与基于微加工的 DMF 相比，基于印刷电路板（PCB）的 DMF 更加经济实惠，并且与现有的 IVD 仪器兼容。尽管如此，目前基于 PCB 的 DMF 往往受到可同时控制的液滴数量的限制，这影响了其通量和作为即时检测工具的应用。在这项工作中，构建了一个可以同时控制多个基于 PCB 的 DMF 板的平台。首先开发了软件和硬件，然后进行了可靠性测试。对基于 PCB 的 DMF 进行了葡萄糖的比色分析，证明了该平台的能力。通过多个板同时操作实现的高通量，这种基于 PCB 的 DMF 有可能为资源有限的环境提供低成本的即时检测。