Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen 518060, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen 518060, China.
Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen 518060, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Medical School, Shenzhen University, Shenzhen 518060, China.
SLAS Technol. 2024 Apr;29(2):100105. doi: 10.1016/j.slast.2023.08.005. Epub 2023 Aug 29.
Digital microfluidics (DMF) has found great applications in vitro diagnostics (IVD). Compared to the microfabrication-based DMF, printed circuit board (PCB)-based DMF is more economical and compatible with existing IVD instruments. Despite that, current PCB-based DMF is oftentimes limited by the available droplets that can be controlled simultaneously, compromising their throughput and applications as point-of-care tools. In this work, a platform that simultaneously controls multiple PCB-based DMF plates was constructed. The software and hardware were first developed, followed by the reliability tests. Colorimetric analysis of glucose was applied to the PCB-based DMF, demonstrating the capability of this platform. With the high throughput enabled by simultaneous operations of multiple plates, this PCB-based DMF can potentially allow point-of-care testing with low cost for resource-limited settings.
数字微流控（DMF）在体外诊断（IVD）中有着广泛的应用。与基于微加工的 DMF 相比，基于印刷电路板（PCB）的 DMF 更加经济实惠，并且与现有的 IVD 仪器兼容。尽管如此，目前基于 PCB 的 DMF 往往受到可同时控制的液滴数量的限制，这影响了其通量和作为即时检测工具的应用。在这项工作中，构建了一个可以同时控制多个基于 PCB 的 DMF 板的平台。首先开发了软件和硬件，然后进行了可靠性测试。对基于 PCB 的 DMF 进行了葡萄糖的比色分析，证明了该平台的能力。通过多个板同时操作实现的高通量，这种基于 PCB 的 DMF 有可能为资源有限的环境提供低成本的即时检测。
