i3N|CENIMAT, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.
UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.
Sensors (Basel). 2017 Jun 25;17(7):1495. doi: 10.3390/s17071495.
Digital Microfluidics (DMF) has emerged as a disruptive methodology for the control and manipulation of low volume droplets. In DMF, each droplet acts as a single reactor, which allows for extensive multiparallelization of biological and chemical reactions at a much smaller scale. DMF devices open entirely new and promising pathways for multiplex analysis and reaction occurring in a miniaturized format, thus allowing for healthcare decentralization from major laboratories to point-of-care with accurate, robust and inexpensive molecular diagnostics. Here, we shall focus on DMF platforms specifically designed for nucleic acid amplification, which is key for molecular diagnostics of several diseases and conditions, from pathogen identification to cancer mutations detection. Particular attention will be given to the device architecture, materials and nucleic acid amplification applications in validated settings.
数字微流控（DMF）已成为一种颠覆性的方法，可用于控制和操纵低体积液滴。在 DMF 中，每个液滴都充当单个反应器，这使得在更小的规模上可以广泛地对生物和化学反应进行多平行化处理。DMF 设备为在微型化格式中发生的多路复用分析和反应开辟了全新的、有前途的途径，从而使医疗保健从主要实验室去中心化到护理点，实现准确、稳健和廉价的分子诊断。在这里，我们将专门关注专为核酸扩增设计的 DMF 平台，这对于几种疾病和病症的分子诊断至关重要，从病原体鉴定到癌症突变检测。将特别关注经过验证的设备架构、材料和核酸扩增应用。
