Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran; Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
J Chem Neuroanat. 2023 Nov;133:102328. doi: 10.1016/j.jchemneu.2023.102328. Epub 2023 Aug 29.
Deep-frying oil (DFO) contains high amounts of free radicals, and consuming foods prepared with this method causes damage to nervous tissue due to oxidative stress (OS). Since moderate-intensity aerobic exercise training (AT) reduces OS, the current search investigated the effects of AT on OS, apoptosis, and neurogenesis markers in the hippocampal tissue of DFO-fed rats. Eighteen Wistar male rats (200-280 gr) were randomly allocated to a control group fed with normal food (Con-ND), a control group receiving DFO (Con-DFO), and a group receiving DFO-aerobic exercise (EX-DFO) (n = 6 in each). DFO was gavaged for four weeks, five days a week, with a dose of 2 ml. AT included running on a treadmill for four weeks and five sessions per week (40 min per session). The expression of genes B-cell lymphoma 2 (BCL-2), Protein X associated with Bcl-2 (BAX), Caspase-3 (Casp-3), and Caspase-9 (Casp-9) was measured by PCR method. The ELISA method was used to calculate levels of Superoxide dismutase (SOD) and Catalase (CAT) activity, malondialdehyde (MDA), and Brain-Derived Neurotrophic Factor (BDNF). Also, the expression of the proteins Cannabinoid receptor type 1(CB1), Cannabinoid receptor type2 (CB2), Glial fibrillary acidic protein (GFAP), Neuronal nuclei (NeuN), and DNA fragmentation was evaluated by Immunohistochemical and TUNEL staining. DFO feeding led to a significant increase in apoptotic markers, such as BAX, Casp-3, and Casp-9 gene expression, and DNA fragmentation (p ≤ 0.05) while decreasing BDNF concentration SOD activity (p ≤ 0.05). AT significantly reduced the BAX, Casp-3, Casp-9, MDA, CB1, GFAP, and DNA fragmentation (p ≤ 0.05). In conclusion, AT can reduce the harmful effects of feeding with DFO on the hippocampal tissue.
深度油炸油（DFO）含有大量自由基，食用用这种方法制备的食物会因氧化应激（OS）而导致神经组织受损。由于中等强度的有氧运动训练（AT）可降低 OS，因此目前的研究调查了 AT 对 DFO 喂养大鼠海马组织中 OS、细胞凋亡和神经发生标志物的影响。18 只 Wistar 雄性大鼠（200-280 克）被随机分配到对照组（喂食正常食物，Con-ND）、对照组（喂食 DFO，Con-DFO）和接受 DFO-有氧运动训练（EX-DFO）的组（每组 6 只）。DFO 灌胃四周，每周 5 天，剂量为 2ml。AT 包括在跑步机上跑步四周，每周 5 次（每次 40 分钟）。通过 PCR 方法测量 B 细胞淋巴瘤 2（BCL-2）、与 Bcl-2 相关的蛋白 X（BAX）、半胱天冬酶-3（Casp-3）和半胱天冬酶-9（Casp-9）基因的表达。ELISA 法用于计算超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性、丙二醛（MDA）和脑源性神经营养因子（BDNF）的水平。此外，还通过免疫组织化学和 TUNEL 染色评估大麻素受体 1（CB1）、大麻素受体 2（CB2）、胶质纤维酸性蛋白（GFAP）、神经元核（NeuN）和 DNA 片段的表达。DFO 喂养导致凋亡标志物（如 BAX、Casp-3 和 Casp-9 基因表达和 DNA 片段）显著增加（p≤0.05），同时 SOD 活性（p≤0.05）和 BDNF 浓度降低。AT 可显著降低 BAX、Casp-3、Casp-9、MDA、CB1、GFAP 和 DNA 片段（p≤0.05）。总之，AT 可以减轻 DFO 喂养对海马组织的有害影响。
