Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Clin Nutr ESPEN. 2022 Jun;49:529-535. doi: 10.1016/j.clnesp.2022.02.008. Epub 2022 Feb 17.
Clinicians who understand how the body responds to exercise, how aerobic training enhances cardiovascular fitness and the benefits and essentials of prescribing aerobic exercise can effectively encourage patients to be active. Deep-frying is a standard cooking method accompanied by the production of carcinogenesis substances such as acrolein. Acrolein is a toxic byproduct of lipid peroxidation involved in the development of pulmonary, cardiac, and neurodegenerative diseases. This study aimed to explore the effect of aerobic exercise (E.X.E.), and octopamine (OCT) on caspase three expression levels in the heart tissue of rats were fed deep-frying oil (D.F.O.).
30 male Wistar rats were divided into 5 groups (n = 6 in each) including (1) control (CO), (2) deep-frying oil (DFO), (3) deep-frying oil + exercise (DFO + EXE), (4) deep-frying oil + octopamine (DFO + OCT), and (5) deep-frying oil + exercise + octopamine (DFO + EXE + OCT). The apoptotic effects of D.F.O. in heart tissue were examined by TUNEL assay. Masson's trichrome stain was used to study cardiomyocytic fibers. Moreover, caspase three gene expression in all groups was evaluated using quantitative real-time PCR and the Western blot method.
Data showed a significant increase in apoptotic cells in the D.F.O. group (P < 0.05). In Masson's Trichrome stain analysis, more cardiomyocytic fibers degradation and lymphocytic aggregation cells in the DFO + EXE + OCT group significantly improve this degradation. Also, the expression level of caspase 3 was significantly decreased in the DFO + EXE + OCT group (P < 0.05).
According to the result of the current study, it can be assumed that D.F.O. can lead to programmed cell death via the activation of caspases in heart tissue. However, it seems that aerobic exercise with octopamine supplementation improves heart tissue function by inhibiting the expression of caspase 3 and pro-caspase 3, leading to a significantly decreased apoptosis in cardiomyocytes of DFO-treated models.
了解身体如何对运动做出反应、有氧训练如何提高心血管健康以及开处有氧运动处方的益处和要点的临床医生，可以有效地鼓励患者积极运动。油炸是一种标准的烹饪方法，伴随着丙烯醛等致癌物质的产生。丙烯醛是脂质过氧化反应的一种有毒副产物，与肺部、心脏和神经退行性疾病的发展有关。本研究旨在探讨有氧运动（E.X.E.）和章鱼胺（OCT）对喂食油炸油（D.F.O.）的大鼠心脏组织中半胱天冬酶 3 表达水平的影响。
将 30 只雄性 Wistar 大鼠分为 5 组（每组 6 只），包括（1）对照组（CO）、（2）油炸油组（DFO）、（3）油炸油+运动组（DFO+EXE）、（4）油炸油+章鱼胺组（DFO+OCT）和（5）油炸油+运动+章鱼胺组（DFO+EXE+OCT）。通过 TUNEL 检测评估 D.F.O.对心脏组织的凋亡作用。Masson 三色染色法用于研究心肌纤维。此外，采用定量实时 PCR 和 Western blot 法检测各组 caspase 3 基因的表达。
数据显示，DFO 组凋亡细胞数量显著增加（P<0.05）。在 Masson 三色染色分析中，DFO+EXE+OCT 组心肌纤维降解和淋巴细胞聚集细胞明显减少，降解程度明显改善。此外，DFO+EXE+OCT 组 caspase 3 的表达水平显著降低（P<0.05）。
根据本研究的结果，可以假设 D.F.O.可以通过激活心脏组织中的半胱天冬酶导致程序性细胞死亡。然而，有氧运动与章鱼胺补充似乎通过抑制 caspase 3 和前半胱天冬酶 3 的表达来改善心脏组织功能，从而显著减少 DFO 处理模型中心肌细胞的凋亡。
