壳聚糖对人鼻中隔软骨来源的软骨细胞的影响。
Effects of Chitosan on Chondrocytes Derived from Human Nasal Septal Cartilage.
机构信息
Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan, R.O.C.
Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, R.O.C.
出版信息
In Vivo. 2023 Sep-Oct;37(5):2001-2005. doi: 10.21873/invivo.13297.
BACKGROUND/AIM: The aim of this study was to evaluate the influence of chitosan on the growth of nasal septal chondrocytes (NSCs). The final goal was to establish a novel methodology to enhance nasal septal cartilage regeneration.
MATERIALS AND METHODS
Human NSCs were isolated and their morphology was examined using Alcian blue staining and observed by light microscopy. The isolated NSCs were grown with various concentrations of chitosan and the expression of COL2A1 was investigated.
RESULTS
NSCs were successfully isolated from nasal septal cartilage. Co-culture with 0.2% of chitosan greatly enhanced proliferation of NSCs compared to control cells. However, 0.5% of chitosan was harmful to NSCs, resulting in cell detachment from the culture plate. Furthermore, the addition of 0.2% chitosan significantly improved the expression of COL2A1 in NSCs.
CONCLUSION
To our knowledge, this is the first report to demonstrate that chitosan could effectively guide the attachment and growth of human NSCs. Chitosan appears to be a promising additive for NSC culture, which sets the stage for studying tissue regeneration in nasal septal cartilage deficiency, rhinoplasty, and craniofacial reconstruction.
背景/目的：本研究旨在评估壳聚糖对鼻中隔软骨细胞（NSCs）生长的影响。最终目标是建立一种增强鼻中隔软骨再生的新方法。
材料和方法
分离人鼻中隔软骨细胞，并用阿利新蓝染色法观察其形态，并通过光学显微镜进行观察。将分离的 NSCs 与不同浓度的壳聚糖一起培养，并研究 COL2A1 的表达。
结果
成功地从鼻中隔软骨中分离出 NSCs。与对照组细胞相比，0.2%壳聚糖的共培养显著促进了 NSCs 的增殖。然而，0.5%的壳聚糖对 NSCs 有害，导致细胞从培养板上脱落。此外，添加 0.2%壳聚糖可显著提高 NSCs 中 COL2A1 的表达。
结论
据我们所知，这是首次报道壳聚糖可有效指导人鼻中隔软骨细胞的附着和生长。壳聚糖似乎是一种有前途的 NSC 培养添加剂，为研究鼻中隔软骨缺陷、鼻整形术和颅面重建中的组织再生奠定了基础。
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