Neumann D A, Tripp M R
Vet Immunol Immunopathol. 1986 Jun;12(1-4):163-74. doi: 10.1016/0165-2427(86)90120-0.
Channel catfish were inoculated intraperitoneally, intramuscularly, or intraesophageally with Yersinia ruckeri. Three antigen doses were administered by each route of injection. Four fish from each treatment were sacrificed at 5-day intervals for 40 days. Serum from each individual was tested for antibody activity against Y. ruckeri by indirect enzyme-linked immunosorbent assay (ELISA). High titers of anti-Y. ruckeri antibody were elicited by all doses (10(5) to 10(9) cells/g of fish) regardless of the route of administration. Mean titers for saline injected fish ranged up to 1:64 for each route of inoculation while mean titers for bacteria injected fish ranged up to 1:4096. Mean titers of 1:128 or greater were observed by day 15 post injection; titers peaked about day 30 and diminished thereafter. Channel catfish were most responsive to the antigen (10(7) to 10(9) cells/g of fish) when administered intramuscularly although lower doses (10(5) to 10(6) cells/g of fish) administered intraperitoneally elicited a substantial response. There was little evidence of dose-dependent responses for any of the routes of immunization. The rapid onset of relatively high serum titers suggests that the fish were mounting a secondary response to Yersinia ruckeri.
用鲁氏耶尔森菌对斑点叉尾鮰进行腹腔内、肌肉内或食管内接种。每种注射途径给予三种抗原剂量。在40天内，每隔5天对每种处理的4条鱼实施安乐死。通过间接酶联免疫吸附测定（ELISA）检测每条鱼血清中针对鲁氏耶尔森菌的抗体活性。无论给药途径如何，所有剂量（10⁵至10⁹个细胞/克鱼）均引发了高滴度的抗鲁氏耶尔森菌抗体。每种接种途径下，注射生理盐水的鱼的平均滴度最高可达1:64，而注射细菌的鱼的平均滴度最高可达1:4096。注射后第15天观察到平均滴度为1:128或更高；滴度在第30天左右达到峰值，此后下降。斑点叉尾鮰肌肉内接种抗原（10⁷至10⁹个细胞/克鱼）时反应最为强烈，不过腹腔内接种较低剂量（10⁵至10⁶个细胞/克鱼）也引发了显著反应。对于任何免疫途径，几乎没有剂量依赖性反应的证据。相对较高的血清滴度迅速出现，表明鱼对鲁氏耶尔森菌产生了二次免疫反应。
