Ingram G A, Alexander J B
Vet Immunol Immunopathol. 1986 Jun;12(1-4):175-80. doi: 10.1016/0165-2427(86)90121-2.
The primary and secondary immune responses were investigated in trout injected with haemocyanin (HCN) intramuscularly (IM) or intraperitoneally (IP), with or without adjuvant. HCN was detected in the blood 30 min after injection and clearance times varied after one injection from 8 to 56 days. Fish given two and three injections cleared the antigen faster. Precipitins against HCN were first detected 21 to 30 days after injection and were still present on Day 56. However, antibodies detectable by indirect haemagglutination (IHA) and complement fixation (CFA) were initially demonstrated 7 to 21 days after a single injection and highest titres were reached between 33 and 56 days according to the experimental protocol. In fish given two injections, maximum titres were reached between Days 42 and 56 (IM), and Days 50 and 56 (IP). With three injections, maximum CFA and IHA values occurred on Days 62 and 66 respectively in the case of IP, and on Days 103 and 106 respectively with IM. Overall, higher titres were found with IHA than by CFA.
研究了虹鳟鱼经肌肉注射（IM）或腹腔注射（IP）接种血蓝蛋白（HCN）后有无佐剂情况下的初次和二次免疫反应。注射后30分钟在血液中检测到HCN，单次注射后的清除时间从8天到56天不等。接受两次和三次注射的鱼清除抗原更快。注射后21至30天首次检测到针对HCN的沉淀素，在第56天时仍存在。然而，通过间接血凝（IHA）和补体结合（CFA）可检测到的抗体在单次注射后7至21天首次出现，根据实验方案，最高滴度在33至56天之间达到。接受两次注射的鱼，在第42至56天（IM）和第50至56天（IP）达到最高滴度。三次注射时，腹腔注射情况下，CFA和IHA的最高值分别出现在第62天和第66天，肌肉注射时分别出现在第103天和第106天。总体而言，IHA检测到的滴度高于CFA。
