Choi Byul-Bora, Choi Jeong-Hae, Kang Tae-Hyung, Lee Seok-Jun, Kim Gyoo-Cheon
Corporate Affiliated Research Institute, Feagle Co., Ltd., Yangsan 50614, Korea.
Onnuri Pain Clinic, Busan 48585, Korea.
Biomedicines. 2021 Oct 26;9(11):1542. doi: 10.3390/biomedicines9111542.
Periodontitis is an inflammatory disease that leads to periodontal tissue destruction and bone resorption. Proliferation and differentiation of cells capable of differentiating into osteoblasts is important for reconstructing periodontal tissues destroyed by periodontitis. In this study, the effects of the nozone (no-ozone) cold plasma (NCP) treatment on osteoblastic differentiation in periodontal ligament (PDL) cells were investigated. To test the toxicity of NCP on PDL cells, various NCP treatment methods and durations were tested, and time-dependent cell proliferation was analyzed using a water-soluble tetrazolium salts-1 assay. To determine the effect of NCP on PDL cell differentiation, the cells were provided with osteogenic media immediately after an NCP treatment to induce differentiation; the cells were then analyzed using alkaline phosphatase (ALP) staining, an ALP activity assay, real time PCR, and Alizarin Red S staining. The NCP treatment without toxicity on PDL cells was the condition of 1-min NCP treatment immediately followed by the replacement with fresh media. NCP increased ALP, osteocalcin, osteonectin, and osteopontin expression, as well as mineralization nodule formation. NCP treatment promotes osteoblastic differentiation of PDL cells; therefore, it may be beneficial for treating periodontitis.
牙周炎是一种导致牙周组织破坏和骨吸收的炎症性疾病。能够分化为成骨细胞的细胞的增殖和分化对于重建因牙周炎而破坏的牙周组织很重要。在本研究中，研究了无臭氧冷等离子体（NCP）处理对牙周膜（PDL）细胞成骨分化的影响。为了测试NCP对PDL细胞的毒性，测试了各种NCP处理方法和持续时间，并使用水溶性四唑盐-1测定法分析了时间依赖性细胞增殖。为了确定NCP对PDL细胞分化的影响，在NCP处理后立即为细胞提供成骨培养基以诱导分化；然后使用碱性磷酸酶（ALP）染色、ALP活性测定、实时PCR和茜素红S染色对细胞进行分析。对PDL细胞无毒性的NCP处理条件是1分钟NCP处理后立即更换新鲜培养基。NCP增加了ALP、骨钙素、骨连接蛋白和骨桥蛋白的表达，以及矿化结节的形成。NCP处理促进了PDL细胞的成骨分化；因此，它可能对治疗牙周炎有益。
