[Antigenic properties of NDV glycoproteins synthesized in the presence of 2-deoxy-d-glucose].

Experiments were carried out to establish whether the glucose antimetabolite, 2-desoxi-d-glucose (DDG), effected the proteolytic breakdown of the envelope proteins of NDV and if there was any effect on there antigenic properties. It was found that it inhibited the glucose action on the NDV envelope proteins, the hemagglutinin-neuraminidase (HN), and the F-protein so that their biologic properties were not manifested, and the production of infectious virus was blocked. In the presence of DDG the virus-infected were shown to contain the virus envelope proteins, the membrane protein, and the nucleocapsid both through electrophoresis in 10 percent polyacrylamide gel and through the agar gel immunodiffusion test (with the use of specific rabbit anti-HN, anti-F protein, and anti-M protein sera). The antigenic properties of the two glycoproteins of the NDV envelope did not change under the action of the DDG inhibitor as seen by the precipitation lines of the control variants (cells infected with the virus but not treated with the antimetabolite), which were identical with those obtained with the test variants (cells treated with DDG).