Hoff J D, Lasley B L, Yen S S
J Clin Endocrinol Metab. 1979 Jul;49(1):8-11. doi: 10.1210/jcem-49-1-8.
To examine the relationship between the priming and releasing actions of LRF on LH secretion, 14 normal cycling women received 4 different rates of LRF infusion (0.005, 0.01, 0.05, and 0.1 microgram/m2.min for 4 h). The releasing action of the infusion was measured as the area under the curve and the priming effect was assessed by the acute LH increment in response to a test pulse of LRF (10 microgram) at the end of infusion. At the lower 2 infusion rates, there were only minor changes in releasing function, but it increased exponentially (r = 0.986) with higher rates of infusion. In contrast, the priming effect of the lower 2 doses of infusion increased markedly as a function of infusion rate, but no additional priming was found with the higher rates of infusion. Thus, over the range of infusion rates employed, the releasing and priming functions of LRF appear to be dose dependent. These results indicate that the interdependent releasing and priming actions of LRF on LH secretion are functionally dissociable and that large elevations of LRF tend to favor release, while small LRF increments seem to promote priming preferentially.
为研究促黄体生成素释放因子（LRF）对促黄体生成素（LH）分泌的启动和释放作用之间的关系，14名正常月经周期的女性接受了4种不同速率的LRF输注（0.005、0.01、0.05和0.1微克/平方米·分钟，持续4小时）。输注的释放作用通过曲线下面积来衡量，启动效应则通过输注结束时对LRF测试脉冲（10微克）的急性LH增量来评估。在较低的2种输注速率下，释放功能仅有轻微变化，但随着输注速率升高呈指数增加（r = 0.986）。相比之下，较低的2种输注剂量的启动效应随输注速率显著增加，但较高输注速率时未发现额外的启动效应。因此，在所采用的输注速率范围内，LRF的释放和启动功能似乎呈剂量依赖性。这些结果表明，LRF对LH分泌的相互依赖的释放和启动作用在功能上是可分离的，且LRF的大幅升高倾向于促进释放，而LRF的小幅增加似乎优先促进启动。
