Ren Kaili, Kong Weiping, Tang Taoxia, Cheng Hong
Vegetable Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, China.
Front Plant Sci. 2023 Aug 15;14:1239482. doi: 10.3389/fpls.2023.1239482. eCollection 2023.
Citric acid is the most important organic acid in melon and has a great influence on fruit flavor quality. ATP-citrate (pro-S) lyase (ACL) is a key regulator in the acetyl-CoA pathway and plays an important role in citric acid metabolism. In this study we analyzed the structure and phylogenetics of genes and their functions in sugar and acid accumulation in melon. A total of four genes were identified in the melon genome, and phylogenetic analysis assigned these genes into the subfamily ( and ) and the subfamily ( and ). Conserved motif and gene structure analyses showed that members of the same subfamily shared identical conserved motifs and gene structures, and probably have similar biological functions. Analysis of -acting elements revealed that promoter sequences contained regulatory elements related to light, stress, phytohormones, and growth and development, indicating that genes may be involved in melon growth and stress responses. The prediction of protein interaction network showed that CmACL proteins were closely related to the proteins belonging to tricarboxylic acid cycle, glyoxylic acid cycle and glycolytic pathway, suggesting that CmACL proteins may play an important role in sugar and acid metabolism. The expression of was significantly and positively correlated with sucrose content, and 2 expression was significantly positively correlated with citric acid content, suggesting that and have important roles in sugar and acid accumulation in melon. Our results offer novel insights and avenues for the regulation of sugar and acid levels in melon and provide a theoretical foundation for breeding high-quality melon cultivars.
柠檬酸是甜瓜中最重要的有机酸，对果实风味品质有很大影响。ATP-柠檬酸（前-S）裂解酶（ACL）是乙酰辅酶A途径中的关键调节因子，在柠檬酸代谢中起重要作用。在本研究中，我们分析了甜瓜中相关基因的结构、系统发育及其在糖和酸积累中的功能。在甜瓜基因组中总共鉴定出四个相关基因，系统发育分析将这些基因分为亚家族（和）和亚家族（和）。保守基序和基因结构分析表明，同一亚家族的成员共享相同的保守基序和基因结构，可能具有相似的生物学功能。对顺式作用元件的分析表明，启动子序列包含与光、胁迫、植物激素以及生长发育相关的调控元件，这表明相关基因可能参与甜瓜的生长和胁迫反应。蛋白质相互作用网络预测表明，CmACL蛋白与属于三羧酸循环、乙醛酸循环和糖酵解途径的蛋白质密切相关，这表明CmACL蛋白可能在糖和酸代谢中起重要作用。的表达与蔗糖含量显著正相关，2的表达与柠檬酸含量显著正相关，这表明和在甜瓜糖和酸积累中具有重要作用。我们的研究结果为调控甜瓜糖酸水平提供了新的见解和途径，并为培育高品质甜瓜品种提供了理论基础。
