Department of Chemistry, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand.
Office of Primary Industries and Mines, Region 3, Ministry of Industry, Chiang Mai, Thailand.
Analyst. 2023 Sep 25;148(19):4753-4761. doi: 10.1039/d3an00879g.
We created novel Pd/CB-Ni@rGO nanomaterials for glucose detection. The as-synthesized nanomaterials were dropped on the electrode surface using the drop casting technique. The prepared electrode was then attached to a paper-based device containing the sample zone and the reaction zone, enabling plasma isolation and an enzymatic reaction for glucose detection in whole blood. The nanomaterials and surfaces of electrodes were characterized by FTIR, TEM, and SEM. The proposed approach is a disposable glucose detection method that is unaffected by protein fouling on the electrode, and it requires only one drop of human blood. Therefore, there is no need for extensive sample preparation, and there is less sample consumption. Under optimal conditions, Pd/CB-Ni@rGO can accurately measure blood glucose levels with a linear range of 7 to 7140 μM ( = 0.9986) and a low detection limit of 0.82 μM. Besides, the developed sensor shows excellent anti-interference capacity, stability, and satisfactory reproducibility and repeatability. Importantly, Pd/CB-Ni@rGO was successfully applied for glucose in whole blood from 4 volunteers, with results that correlated well with those obtained using an Accucheck glucometer at a 95% confidence level. Given its low cost, high accuracy, and ease of use, the blood glucose sensor holds significant potential for clinical use and broadens the area of future noninvasive sensor development.
我们制备了新型 Pd/CB-Ni@rGO 纳米材料用于葡萄糖检测。采用滴涂技术将合成的纳米材料滴到电极表面。然后将制备好的电极连接到一个基于纸张的装置上，该装置包含样品区和反应区，实现等离子体隔离和全血中葡萄糖的酶反应检测。纳米材料和电极表面通过傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）和扫描电子显微镜（SEM）进行了表征。所提出的方法是一种一次性的葡萄糖检测方法，不受电极上蛋白质污染的影响，只需要一滴人血。因此，不需要进行广泛的样品制备，也减少了样品的消耗。在最佳条件下，Pd/CB-Ni@rGO 可以准确测量 7 至 7140 μM 的线性范围（ = 0.9986），检测限低至 0.82 μM。此外，开发的传感器具有出色的抗干扰能力、稳定性以及令人满意的重现性和可重复性。重要的是，Pd/CB-Ni@rGO 成功应用于来自 4 位志愿者的全血中的葡萄糖检测，结果与 Accucheck 血糖仪在 95%置信水平下的检测结果具有良好的相关性。鉴于其低成本、高准确性和易用性，血糖传感器具有很大的临床应用潜力，并拓宽了未来无创传感器发展的领域。
