LytS 型组氨酸激酶 BtsS 是一种 7 次跨膜受体，可结合丙酮酸。

The LytS-type histidine kinase BtsS is a 7-transmembrane receptor that binds pyruvate.

机构信息

Faculty of Biology, Microbiology, Ludwig-Maximilians-Universität München , Martinsried, Germany.

Center of Functional Protein Assemblies, Technical University of Munich , Garching, Germany.

出版信息

mBio. 2023 Oct 31;14(5):e0108923. doi: 10.1128/mbio.01089-23. Epub 2023 Sep 1.

DOI:10.1128/mbio.01089-23

PMID:37655896

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10653868/

Abstract

Here, we studied the LytS-type histidine kinase BtsS of and identified the pyruvate binding site within the membrane-spanning domains. It is a small cavity, and pyruvate forms interactions with the side chains of Arg72, Arg99, Cys110, and Ser113 located in transmembrane helices III, IV, and V, respectively. Our results can serve as a starting point to convert BtsS into a sensor for structurally similar ligands such as lactate, which can be used as biosensor in medicine.

摘要

在这里，我们研究了中的 LytS 型组氨酸激酶 BtsS，并确定了跨膜结构域内的丙酮酸结合位点。这是一个小空腔，丙酮酸分别与位于跨膜螺旋 III、IV 和 V 中的 Arg72、Arg99、Cys110 和 Ser113 的侧链形成相互作用。我们的研究结果可以作为将 BtsS 转化为类似结构配体（如乳酸）传感器的起点，这种传感器可用于医学中的生物传感器。