Wang Xing, He Shan-Wen, He Qing, Ju Zhi-Cheng, Ma Yi-Nan, Wang Zhe, Han Jia-Cheng, Zhang Xiao-Xia
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences , Beijing, China.
Shanghai Academy of Landscape Architecture Science and Planning , Shanghai, China.
Microbiol Spectr. 2023 Sep 1;11(5):e0497822. doi: 10.1128/spectrum.04978-22.
The core endophytes of plants are regarded as promising resources in future agroecosystems. How they affect the assembly of rice-related bacterial communities after early inoculation remains unclear. Here, we examined bacterial communities across 148 samples, including bulk and rhizosphere soils, sterilized roots, stems, and seeds at the seedling, tillering, booting, and maturity stages. Tissue cultured rice seedlings were inoculated with JR3-14, a core endophytic bacterium of rice seeds, before transplanting. The results revealed that α-diversity indices were significantly enhanced in the root and stem endosphere at the seedling stage. β-diversity was altered at most plant developmental stages, except for the root and stem at the booting stage. Network complexity consequently increased in the root and stem across rice growth stages, other than the stem endosphere at the booting stage. Four abundant beneficial bacterial taxa, , , , and , were co-enriched during the early growth stage. Infer Community Assembly Mechanisms by Phylogenetic-bin-based null model analysis revealed a higher relative contribution of drift and other eco-evolutionary processes mainly in root compartments across all growth stages, but the opposite pattern was observed in stem compartments. IMPORTANCE Endophytic bacteria are regarded as promising environmentally friendly resources to promote plant growth and plant health. Some of microbes from the seed are able to be carried over to next generation, and contribute to the plant's ability to adapt to new environments. However, the effects of early inoculation with core microbes on the assembly of the plant microbiome are still unclear. In our study, we demonstrate that early inoculation of the rice seed core endophytic bacterium could alter community diversity, enhance complexity degree of network structure at most the growth stages, and enrich beneficial bacteria at the seedling stage of rice. We further analyzed the evolutionary processes caused by the early inoculation. Our results highlight the new possibilities for research and application of sustainable agriculture by considering the contribution of seed endophytes in crop production and breeding.
植物的核心内生菌被视为未来农业生态系统中很有前景的资源。早期接种后它们如何影响与水稻相关的细菌群落组装仍不清楚。在这里，我们检测了148个样本中的细菌群落，包括在幼苗期、分蘖期、孕穗期和成熟期的土壤、根际土壤、消毒后的根、茎和种子。在移栽前，用水稻种子的核心内生细菌JR3-14接种组织培养的水稻幼苗。结果表明，在幼苗期，根和茎的内生菌中α-多样性指数显著提高。除孕穗期的根和茎外，在大多数植物发育阶段β-多样性都发生了改变。因此，除孕穗期的茎内生菌外，在水稻生长阶段，根和茎中的网络复杂性增加。在早期生长阶段，四种丰富的有益细菌类群，即 、 、 和 共同富集。通过基于系统发育箱的空模型分析推断群落组装机制表明，在所有生长阶段，漂移和其他生态进化过程的相对贡献在根部隔室中更高，但在茎部隔室中观察到相反的模式。重要性 内生细菌被视为促进植物生长和植物健康的有前景的环境友好型资源。种子中的一些微生物能够传递到下一代，并有助于植物适应新环境的能力。然而，早期接种核心微生物对植物微生物组组装的影响仍不清楚。在我们的研究中，我们证明早期接种水稻种子核心内生细菌可以改变群落多样性，在大多数生长阶段提高网络结构的复杂程度，并在水稻幼苗期富集有益细菌。我们进一步分析了早期接种引起的进化过程。我们的结果突出了通过考虑种子内生菌在作物生产和育种中的贡献，为可持续农业研究和应用带来的新可能性。
