Ma Jiejia, Peng Qianze, Chen Silu, Liu Zhuoxin, Zhang Weixing, Zhang Chi, Du Xiaohua, Sun Shue, Peng Weiye, Lei Ziling, Zhang Limei, Su Pin, Zhang Deyong, Liu Yong
Longping Branch, College of Biology, Hunan University, Changsha 410082, China.
State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Academy of Agricultural Sciences, Changsha 410125, China.
Microorganisms. 2025 Apr 20;13(4):947. doi: 10.3390/microorganisms13040947.
The interactions between plants and microbes are essential for enhancing crop productivity. However, the mechanisms underlying host-specific microbiome migration and functional assembly remain poorly understood. In this study, microbiome migration from soil to leaves in rice () and maize () was analyzed through 16S rRNA sequencing and phenotypic assessments. When we used the same soil microbiome source to grow rice and maize, microbiota and functional traits were specifically enriched by maize in its phyllosphere and rhizosphere. This indicated that plants can selectively assemble microbiomes from a shared microbiota source. Therefore, 22 strains were isolated from the phyllospheres of rice and maize and used to construct a synthetic microbial community (SynCom). When the soil for rice and maize growth was inoculated with the SynCom, strains belonging to were enriched in the maize phyllosphere compared to the rice phyllosphere. Additionally, a strain belonging to was enriched in the maize rhizosphere compared to the rice rhizosphere. These results suggest that plant species influence the migration of microbiota within their respective compartments. Compared with mock inoculation, SynCom inoculation significantly enhanced plant growth. When we compared the microbiomes, strains belonging to , which were assembled by both rice and maize, played a role in enhancing plant growth. Our findings underscore the importance of microbial migration dynamics and functional assembly in leveraging plant-microbe interactions for sustainable agriculture.
植物与微生物之间的相互作用对于提高作物生产力至关重要。然而，宿主特异性微生物组迁移和功能组装的潜在机制仍知之甚少。在本研究中，通过16S rRNA测序和表型评估分析了水稻（）和玉米（）中微生物组从土壤到叶片的迁移。当我们使用相同的土壤微生物组来源种植水稻和玉米时，玉米在其叶际和根际中特异性富集了微生物群和功能性状。这表明植物可以从共享的微生物群来源中选择性地组装微生物组。因此，从水稻和玉米的叶际中分离出22株菌株，并用于构建合成微生物群落（SynCom）。当用SynCom接种水稻和玉米生长的土壤时，与水稻叶际相比，属于的菌株在玉米叶际中富集。此外，与水稻根际相比，属于的一株菌株在玉米根际中富集。这些结果表明植物物种会影响其各自区内微生物群的迁移。与模拟接种相比，SynCom接种显著促进了植物生长。当我们比较微生物组时，由水稻和玉米共同组装的属于的菌株在促进植物生长方面发挥了作用。我们的研究结果强调了微生物迁移动态和功能组装在利用植物 - 微生物相互作用实现可持续农业方面的重要性。
