Institute of Microbiology, Faculty of Life Sciences, Government College University Faisalabad, 38000, Pakistan.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Saudi Arabia.
Future Microbiol. 2023 Sep;18:959-969. doi: 10.2217/fmb-2023-0068. Epub 2023 Sep 1.
To determine the prevalence of antimicrobial-resistant genes in carbapenem-resistant (CRECO). A total of 290 carbapenem-resistant bacteria were collected from tertiary care hospitals in Lahore (Pakistan). These isolates were confirmed by VITEK 2 and matrix-assisted laser desorption/ionization time of flight. The minimum inhibitory concentration was performed by VITEK 2. Sequence typing, resistant gene identification, DNA hybridization and replicate typing were also performed. 33 out of 290 (11.3%) were CRECO and carried ; 69, 18 and 12% were NDM-1, NDM-5 and NDM-7, respectively, with 100% resistance to β-lactams and β-lactam inhibitors. ST405 and ST468 were mostly identified. NDM-ECO carried approximately 50-450 kb of plasmids and 16 (55%) were associated with IncA/C. NDM-1-producing are highly prevalent in clinical settings.
为了确定耐碳青霉烯肠杆菌科（CRECO）中抗微生物耐药基因的流行率。从巴基斯坦拉合尔的三级保健医院共收集了 290 株耐碳青霉烯细菌。这些分离株通过 VITEK 2 和基质辅助激光解吸/电离飞行时间进行了确认。通过 VITEK 2 进行了最低抑菌浓度测定。还进行了序列分型、耐药基因鉴定、DNA 杂交和重复分型。290 株中的 33 株（11.3%）为 CRECO，携带 ; 分别为 NDM-1、NDM-5 和 NDM-7，分别为 69、18 和 12%，对β-内酰胺类和β-内酰胺抑制剂有 100%的耐药性。主要鉴定出 ST405 和 ST468。NDM-ECO 携带约 50-450kb 的质粒，其中 16 个（55%）与 IncA/C 相关。产 NDM-1 的 在临床环境中非常普遍。
