Max Planck Unit for the Science of Pathogens, D-10117 Berlin, Germany.
Center for Human Technologies, Istituto Italiano di Tecnologia, 16152 Genova, Italy.
FEMS Microbiol Rev. 2023 Sep 5;47(5). doi: 10.1093/femsre/fuad049.
Bacteria adjust gene expression at the post-transcriptional level through an intricate network of small regulatory RNAs and RNA-binding proteins, including ribonucleases (RNases). RNases play an essential role in RNA metabolism, regulating RNA stability, decay, and activation. These enzymes exhibit species-specific effects on gene expression, bacterial physiology, and different strategies of target recognition. Recent advances in high-throughput RNA sequencing (RNA-seq) approaches have provided a better understanding of the roles and modes of action of bacterial RNases. Global studies aiming to identify direct targets of RNases have highlighted the diversity of RNase activity and RNA-based mechanisms of gene expression regulation. Here, we review recent RNA-seq approaches used to study bacterial RNases, with a focus on the methods for identifying direct RNase targets.
细菌通过复杂的小调控 RNA 和 RNA 结合蛋白（包括核糖核酸酶 (RNase)）网络在转录后水平上调节基因表达。RNase 在 RNA 代谢中发挥着重要作用，调节 RNA 的稳定性、降解和激活。这些酶对基因表达、细菌生理学和不同的靶标识别策略具有特定的物种效应。高通量 RNA 测序 (RNA-seq) 方法的最新进展提供了对细菌 RNase 的作用和作用模式的更好理解。旨在确定 RNase 直接靶标的全局研究强调了 RNase 活性和基于 RNA 的基因表达调控机制的多样性。在这里，我们回顾了最近用于研究细菌 RNase 的 RNA-seq 方法，重点介绍了鉴定直接 RNase 靶标的方法。
