采用卵清蛋白和不同密度硫酸半酯多糖纳米载体制备花色苷纳米复合物：提高稳定性、生物可及性和抗氧化性能。
Fabrication of nanocomplexes for anthocyanins delivery by ovalbumin and differently dense sulphate half-ester polysaccharides nanocarriers: Enhanced stability, bio-accessibility, and antioxidant properties.
机构信息
Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China.
College of Food Science, Shenyang Agricultural University, National R&D Professional Center For Berry Processing, National Engineering and Technology of Research Center For Small Berry, Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing, Liaoning Province, Shenyang, Liaoning 110866, China.
出版信息
Food Chem. 2024 Jan 30;432:137263. doi: 10.1016/j.foodchem.2023.137263. Epub 2023 Aug 25.
This study aimed to fabricate novel nanocomplexes for delivery of anthocyanins (ACN) utilizing ovalbumin (OVA) and sulphated-polysaccharides with varying linear charge density (κ-,ι-, λ-carrageenan and dextran sulfate: κC < ιC < λC < DS). Influence of OVA-sulphated-polysaccharides on ACN stability, antioxidant capacity, and bioaccessibility was investigated. Fabricated nanoparticlecosmeticsed superior encapsulation efficiency (94.11-96.2%) and loaded capacity (9.05-9.54%) for ACN. OVA-DS displayed the smallest particle size and turbidity, while OVA-κC-ACN exhibited the largest ones. ζ-Potential of nanoparticles raised with increasing ester-sulfate level in sulphated-polysaccharides. FT-IR, Raman and OVA conformational alterations revealed existence of intermolecular-interactions between ACN and OVA-polysaccharides. DSC and TGA showed considerable thermo-stability of self-assembled (ACN-loaded) OVA-polysaccharides. Spheroid-nanoparticles size increased after ACN-loading in SEM and CLSM. Composite nanocomplexes enhanced ACN stability and antioxidant properties under accelerated degradation conditions and simulated digestion, particularly, OVA-DS-ACN and OVA-λC-ACN. We provide a choice for reinforcing stability of hydrophilic nutraceuticals and improving its applications.
本研究旨在利用卵清蛋白（OVA）和具有不同线电荷密度（κ-、ι-、λ-卡拉胶和葡聚糖硫酸酯：κC < ιC < λC < DS）的硫酸化多糖来制备新型纳米复合物以递送花色苷（ACN）。研究了 OVA-硫酸化多糖对 ACN 稳定性、抗氧化能力和生物利用度的影响。所制备的纳米颗粒化妆品具有优异的 ACN 包封效率（94.11-96.2%）和负载能力（9.05-9.54%）。OVA-DS 表现出最小的粒径和浊度，而 OVA-κC-ACN 则表现出最大的粒径和浊度。纳米颗粒的 ζ-电位随硫酸化多糖中酯硫酸基水平的增加而升高。FT-IR、拉曼和 OVA 构象变化表明 ACN 与 OVA-多糖之间存在分子间相互作用。DSC 和 TGA 表明自组装（负载 ACN 的）OVA-多糖具有相当的热稳定性。在 SEM 和 CLSM 中，球形体纳米颗粒的尺寸在负载 ACN 后增加。复合纳米复合物在加速降解条件和模拟消化下增强了 ACN 的稳定性和抗氧化性能，特别是 OVA-DS-ACN 和 OVA-λC-ACN。我们为增强亲水性营养保健品的稳定性和改善其应用提供了一种选择。
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