College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
Food Funct. 2018 Jul 17;9(7):3788-3797. doi: 10.1039/c8fo00300a.
The objective of this study was to investigate the impact of encapsulating resveratrol in ovalbumin (OVA)-carboxymethylcellulose (CMC) nanocomplexes or nanoparticles on its photostability and bioaccessibility. The nanocomplexes were formed by the electrostatic assembly between OVA and CMC, and the nanoparticles were fabricated through further heating the nanocomplexes at 90 °C for 30 min. The nanocomplexes and nanoparticles both presented a spherical morphology, and the nanoparticles exhibited larger average particle sizes and net zeta potential. The encapsulation efficiency and loading capacity were around 26% and 13 μg mg-1 for OVA/CMC nanocomplexes, and evidently increased to around 70% and 35 μg mg-1 for OVA/CMC nanoparticles, respectively. The major driving forces for resveratrol encapsulation were hydrogen bonding and hydrophobic interaction. Both the formulations could improve the photostability of trans-resveratrol when exposed to UV light, and the nanoparticles were more effective. Moreover, the release profile of resveratrol from the nanocapsules in the in vitro simulated gastrointestinal tract was greatly enhanced and could be well fitted using the Higuchi kinetic model and Korsmeyer-Peppas model, indicating a Fickian diffusion release mechanism. Compared to native resveratrol, the bioaccessibility of resveratrol embedded in nanocomplexes and nanoparticles was increased to 60% and 80%, respectively. These findings suggest that OVA/CMC nanocomplexes and nanoparticles have potential applications in the development of effective oral delivery systems of resveratrol as well as other lipophilic nutraceuticals into functional foods.
本研究旨在探究将白藜芦醇包埋于卵清蛋白（OVA）-羧甲基纤维素（CMC）纳米复合物或纳米颗粒中对其光稳定性和生物利用度的影响。纳米复合物通过 OVA 与 CMC 之间的静电组装形成，纳米颗粒则是通过进一步将纳米复合物在 90°C 下加热 30 分钟制备得到。纳米复合物和纳米颗粒均呈现出球形形态，且纳米颗粒具有更大的平均粒径和净 ζ 电位。OVA/CMC 纳米复合物的包封效率和载药量约为 26%和 13 μg mg-1，而 OVA/CMC 纳米颗粒的包封效率和载药量明显增加到约 70%和 35 μg mg-1。白藜芦醇包封的主要驱动力为氢键和疏水相互作用。两种制剂均可提高在紫外光下暴露的反式白藜芦醇的光稳定性，且纳米颗粒的效果更为显著。此外，在体外模拟胃肠道中，白藜芦醇从纳米胶囊中的释放曲线得到了极大改善，并且可以使用 Higuchi 动力学模型和 Korsmeyer-Peppas 模型进行很好地拟合，表明其为菲克扩散释放机制。与天然白藜芦醇相比，包埋于纳米复合物和纳米颗粒中的白藜芦醇的生物利用度分别提高至 60%和 80%。这些发现表明，OVA/CMC 纳米复合物和纳米颗粒在开发有效口服递送系统以将白藜芦醇及其他脂溶性营养保健品递送至功能性食品中具有潜在应用价值。
