College of Animal Science and Technology, Northwest A&F University, Yangling, China.
College of Grassland Agriculture, Northwest A&F University, Yangling, China.
Gene. 2023 Dec 20;888:147750. doi: 10.1016/j.gene.2023.147750. Epub 2023 Aug 30.
The Janus kinase/signal transducer and transporter activator (JAK/STAT) signaling pathway plays crucial roles in lipid metabolism, glucose metabolism and cell senescence, suggesting that they are potential candidate genes affecting growth traits in animals. The present study aimed to evaluate the association between InDels in the JAK/STAT pathway and growth traits of four Chinese sheep breeds, including Tong sheep, Hu sheep, Small-tailed Han sheep and Lanzhou fat-tailed sheep.
Seventy-six indel loci of 11 genes in JAK/STAT were detected, and three genotypes were selected at four loci by PCR amplification, electrophoresis and sequencing, including one locus in STAT3, one locus in STAT5A, and two loci in JAK1. The Correlation analysis indicated that there was no significant correlation between STAT3 and growth traits in four sheep breeds (P > 0.05); STAT5A was significantly associated with body height, rump width and tube circumference in Hu sheep and body length in Tong sheep (P < 0.05); JAK1 was significantly correlated with body height, body oblique length, cross height and tube circumference in Hu sheep (P < 0.05) and body oblique length, cross height and tube circumference in small-tailed Han sheep (P < 0.05).
Overall, our results indicated a potential association between the growth traits of sheep and the InDels of JAK1 and STAT5A.
Janus 激酶/信号转导和转录激活因子（JAK/STAT）信号通路在脂质代谢、葡萄糖代谢和细胞衰老中发挥着重要作用，这表明它们是影响动物生长性状的潜在候选基因。本研究旨在评估 JAK/STAT 通路中的 InDels 与中国四个绵羊品种（滩羊、湖羊、小尾寒羊和兰州大尾羊）生长性状之间的关系。
检测到 JAK/STAT 中 11 个基因的 76 个插入缺失位点，并通过 PCR 扩增、电泳和测序在四个位点选择了三种基因型，包括 STAT3 中的一个位点、STAT5A 中的一个位点和 JAK1 中的两个位点。相关性分析表明，STAT3 与四个绵羊品种的生长性状之间无显著相关性（P>0.05）；STAT5A 与湖羊的体高、臀宽和管围以及滩羊的体长显著相关（P<0.05）；JAK1 与湖羊的体高、体斜长、胸宽和管围（P<0.05）以及小尾寒羊的体斜长、胸宽和管围（P<0.05）显著相关。
总体而言，本研究结果表明绵羊生长性状与 JAK1 和 STAT5A 的 InDels 之间存在潜在关联。
