a College of Animal Science and Technology , Northwest A&F University , Yangling , China.
b Science Experimental Center , Northwest University for Nationalities , Lanzhou , China.
Anim Biotechnol. 2018;29(4):276-282. doi: 10.1080/10495398.2017.1379415. Epub 2017 Dec 4.
In China, Tong sheep (TS) and Lanzhou fat-tailed sheep (LFTS) are two closely relative endanger breeds for low meat production and low fecundity, finding some marker-assisted selected (MAS) is our first priority for improving their growth traits. For this purpose, Hu sheep (HS) and small-tailed Han sheep (STHS) were compared with two endangered breeds (TS and LFTS). Paired-liked homeodomain transcription factor 2 (PITX2) gene was the important member of PITX family, which could adjust animal growth through hypothalamic-pituitary-adrenal axis. During the past years, insertion/deletion (indel) has become increasingly popular in application as MAS. In this study, two novel indel loci were identified, and five significant differences, including chest width, hip width, chest depth, chest circumference, and body height, were found between different breeds. Interestingly, there was no DD genotype and smaller number of ID genotye. All the ID genotypes were significantly greater than II genotype, which was to say the allele of "D" was dominant variation and its frequency was lower, which demonstrated that it has huge space for selection. Briefly, the two indel were potential and useful DNA markers for selecting excellent individuals in relation to growth traits in sheep.
在中国，滩羊（TS）和兰州大尾羊（LFTS）是两种亲缘关系密切的濒危品种，由于产肉量低和繁殖力低，我们首先需要找到一些标记辅助选择（MAS）的方法来提高它们的生长性状。为此，我们将湖羊（HS）和小尾寒羊（STHS）与这两个濒危品种（TS 和 LFTS）进行了比较。同源异型盒基因 PITX2 是 PITX 家族的重要成员，它可以通过下丘脑-垂体-肾上腺轴来调节动物的生长。在过去的几年中，插入/缺失（indel）作为 MAS 越来越受欢迎。在这项研究中，我们鉴定了两个新的 indel 位点，并发现了五个显著的差异，包括胸围、臀围、胸深、胸围和体高，这些差异存在于不同的品种之间。有趣的是，在不同品种中，没有发现 DD 基因型和较小数量的 ID 基因型。所有的 ID 基因型都显著大于 II 基因型，这意味着“D”等位基因是显性变异，其频率较低，这表明它有很大的选择空间。总之，这两个 indel 是与绵羊生长性状相关的潜在有用的 DNA 标记，可以用于选择优秀个体。
