Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland, USA.
Department of Biology and CESAM, University of Aveiro, Aveiro, Portugal.
J Eukaryot Microbiol. 2024 Jan-Feb;71(1):e12998. doi: 10.1111/jeu.12998. Epub 2023 Sep 1.
The Blastocystis subtype ST10 has been recognized to contain a great deal of diversity at the sequence level, potentially indicating the presence of multiple new STs within the clade. However, the data needed to validate these new STs were not available. To help resolve this diversity, full-length small subunit (SSU) rRNA gene reference sequences were generated using Oxford Nanopore MinION long-read sequencing from 21 samples representing multiple domestic and wild hosts and geographic regions and covering the sequence diversity previously described using fragments of the SSU rRNA gene. Phylogenetic and pairwise distance analyses were used to compare full-length sequences of the SSU rRNA gene generated in this study with all other valid STs of Blastocystis. We present data supporting the division of ST10/ST23 cluster into five subtypes, ST10, ST23, and three new subtypes with the proposed ST designations of ST42, ST43, and ST44. As the host range of Blastocystis continues to expand with new subtypes and new hosts being frequently identified, the reference sequences provided in this study will assist in accurate sequence classification and help to clarify the epidemiology of this common intestinal microeukaryote.
已确认 Blastocystis 亚型 ST10 在序列水平上存在大量多样性，这可能表明该进化枝内存在多个新的 ST。然而，验证这些新 ST 所需的数据尚不可用。为了帮助解决这种多样性，使用 Oxford Nanopore MinION 长读测序技术，从代表多个家养和野生宿主以及涵盖先前使用 SSU rRNA 基因片段描述的序列多样性的 21 个样本中生成了全长小亚基 (SSU) rRNA 基因参考序列。系统发育和成对距离分析用于比较本研究中生成的 SSU rRNA 全长序列与 Blastocystis 的所有其他有效 ST。我们提供的数据支持将 ST10/ST23 簇分为五个亚型，即 ST10、ST23 和三个新亚型，建议的 ST 命名为 ST42、ST43 和 ST44。随着 Blastocystis 的宿主范围不断扩大，新的亚型和新的宿主经常被发现，本研究提供的参考序列将有助于准确的序列分类，并有助于阐明这种常见的肠道微真核生物的流行病学。
