从沙捞越（马来西亚婆罗洲）的棕果蝠（Penthetor lucasi）中检测到新型冠状病毒。

Detection of novel coronaviruses from dusky fruit bat (Penthetor lucasi) in Sarawak, Malaysian Borneo.

机构信息

Center for Tropical and Emerging Diseases, Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak, Kota Samarahan, Sarawak, Malaysia.

Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Kota Samarahan, Sarawak, Malaysia.

出版信息

Vet Med Sci. 2023 Nov;9(6):2634-2641. doi: 10.1002/vms3.1251. Epub 2023 Sep 2.

DOI:10.1002/vms3.1251

PMID:37658663

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10650259/

Abstract

BACKGROUND

Sarawak has one of the highest diversity of fruit bats species (family Pteropodidae) in Malaysia, with 19 species described. Most coronavirus (CoV) studies have mainly focused on insectivorous bats, resulting in a lack of information on CoVs present in frugivorous bats. In addition, bat CoV surveillance activities are lacking in Malaysia.

OBJECTIVES

Our study focuses on determining the presence of bat CoVs in dusky fruit bat (Penthetor lucasi).

METHODS

Guano samples belonging to P. lucasi were collected from Wind Cave Nature Reserve. The samples were screened for the presence of CoVs using validated hemi-nested consensus RNA-dependent RNA polymerase consensus primers.

RESULTS

The bat CoV positivity rate was 38.5% (n = 15/39), with the viruses belonging to two subgenera: Alphacoronavirus (α-CoV) and Betacoronavirus (β-CoV). Phylogenetic analysis revealed that CoVs from 14 samples of P. lucasi belong to the genus α-CoV and may represent previously described genetic lineages in insectivorous bats in Wind Cave. However, only one sample of P. lucasi was detected with β-CoV which is closely related to subgenus Nobecovirus, which is commonly seen in frugivorous bats.

CONCLUSIONS

This study provides the first available data on CoVs circulating in P. lucasi.

摘要

背景

沙捞越拥有马来西亚种类最多的果蝠（翼手目）之一，有 19 种已被描述。大多数冠状病毒（CoV）研究主要集中在食虫蝙蝠上，因此对食果蝙蝠中存在的 CoV 知之甚少。此外，马来西亚缺乏对蝙蝠 CoV 的监测活动。

目的

我们的研究重点是确定暗棕果蝠（Penthetor lucasi）中是否存在蝙蝠 CoV。

方法

从风洞自然保护区采集暗棕果蝠的粪便样本。使用经过验证的半巢式套式 RNA 依赖性 RNA 聚合酶套式共识引物，对样本进行 CoV 存在情况的筛查。

结果

蝙蝠 CoV 的阳性率为 38.5%（n=15/39），病毒属于两个亚属：α-冠状病毒（α-CoV）和β-冠状病毒（β-CoV）。系统进化分析显示，来自 14 份暗棕果蝠样本的 CoV 属于α-CoV 属，可能代表了在风洞食虫蝙蝠中已描述的遗传谱系。然而，只有一份暗棕果蝠样本检测出β-CoV，它与诺布科病毒亚属密切相关，这种病毒常见于食果蝙蝠中。