Bi Liangliang, Teng Yue, Baghayeri Mehdi, Bao Jinlei
Department of Ultrasound Diagnosis, Affiliated Hospital of Hebei University, Baoding, 071000, Hebei, China.
Faculty of Medicine, Health and Life Science, Swansea University, SA2 8PP, Swansea, Wales, UK.
Environ Res. 2023 Nov 15;237(Pt 2):117030. doi: 10.1016/j.envres.2023.117030. Epub 2023 Aug 31.
An effective biosensing platform is described based on halloysite nanotube/carbon composite decorated with Pd nanoparticles (HNT/C@Pd NPs). A novel electrochemical aptasensor was designed using the proposed nano-platform to determine human epidermal growth factor receptor 2 (HER2), a breast cancer biomarker. Inherently, aptasensing interfaces provide high sensitivity and selectivity for tumor markers owing to the high specific surface area of HNT/C and good conductivity stems from deposition of Pd NPs into HNT/C composite. With a correlation coefficient of 0.996, the electrochemical aptasensor demonstrated a wide linear range from 0.03 ng/mL to 9 ng/mL. The limit of detection (LOD) of the established assay was 8 pg/mL based on S/N = 3 method. Further, the designed biosensor demonstrated acceptable selectivity, good reproducibility, and high stability. The applicability of the impedimetric sensor in human serum samples was also examined and compared to enzyme-linked immunosorbent assay (ELISA) assay (p-value >0.05). Based on the results, it was found that the proposed methodology can be used in quantification of breast cancer markers for early diagnosis and treatment.
本文描述了一种基于用钯纳米颗粒修饰的埃洛石纳米管/碳复合材料（HNT/C@Pd NPs）的有效生物传感平台。利用该纳米平台设计了一种新型电化学适体传感器，用于检测乳腺癌生物标志物人表皮生长因子受体2（HER2）。本质上，由于HNT/C的高比表面积以及钯纳米颗粒沉积到HNT/C复合材料中带来的良好导电性，适体传感界面为肿瘤标志物提供了高灵敏度和选择性。该电化学适体传感器的相关系数为0.996，线性范围为0.03 ng/mL至9 ng/mL。基于S/N = 3的方法，所建立检测方法的检测限为8 pg/mL。此外，所设计的生物传感器表现出可接受的选择性、良好的重现性和高稳定性。还研究了该阻抗传感器在人血清样本中的适用性，并与酶联免疫吸附测定（ELISA）进行比较（p值>0.05）。基于这些结果，发现所提出的方法可用于乳腺癌标志物的定量分析，以实现早期诊断和治疗。
