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[使用免疫沉淀剂制备抗乳过氧化物酶单特异性抗血清]

[Production of a monospecific antiserum to lactoperoxidase using immunoprecipitants].

作者信息

Denisova I I

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1986 Aug(8):88-90.

PMID:3765979
Abstract

To prepare monospecific antiserum to lactoperoxidase, one of the factors of the nonspecific protection of the body from infections, the complexes of lactoperoxidase with antibodies to it were used as antigens; precipitates were obtained by linear immunoelectrophoresis with the use of partially purified lactoperoxidase and antisera to it. This method does not require obtaining the highly purified enzyme and the subsequent adsorption of antisera.

摘要

为制备针对乳铁蛋白(机体抗感染非特异性保护因子之一)的单特异性抗血清,使用乳铁蛋白与其抗体的复合物作为抗原;通过线性免疫电泳,利用部分纯化的乳铁蛋白及其抗血清获得沉淀物。该方法无需获得高度纯化的酶以及后续对抗血清进行吸附。

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