Kroll J, Andersen M M
J Immunol Methods. 1976;13(2):125-30. doi: 10.1016/0022-1759(76)90150-2.
Individual plasma proteins were precipitated, identified and isolated on the basis of line immunoelectrophoresis. A monospecific antibody response was induced by immunization of rabbits with less than 50 ng of precipitated antigen. Preservation of monospecificity was obtained by reimmunization with precipitates developed against the specific antisera.
基于线状免疫电泳对个体血浆蛋白进行沉淀、鉴定和分离。用少于50纳克的沉淀抗原免疫兔子可诱导产生单特异性抗体反应。通过用针对特异性抗血清产生的沉淀物进行再次免疫来保持单特异性。