Department of Microbiology, The Nippon Dental University School of Life Dentistry at Tokyo, Tokyo, Japan.
J Oral Biosci. 2023 Dec;65(4):273-279. doi: 10.1016/j.job.2023.08.008. Epub 2023 Sep 1.
Porphyromonas gingivalis is the etiological agent of chronic periodontitis. Menadione (vitamin K) and phylloquinone (vitamin K) are well-known growth factors for P. gingivalis, while menadione is widely used in growth experiments. Here we attempted to determine the differences in phylloquinone and menadione in P. gingivalis growth experiments, which have not been well studied to date.
We investigated the effects of menadione and phylloquinone on the growth of two W83 strains and seven ATCC 33277 strains of P. gingivalis.
The ATCC 33277 strains grew well with phylloquinone at 2.9 μM in a complex medium (nutrient medium) and at 29 μM in two minimal media. In contrast, the W83 strains grew well without menadione or phylloquinone in three different culture media. Menadione at 2.9 μM, the conventionally used concentration for culturing P. gingivalis, supported the growth of most ATCC 33277 strains but inhibited the growth of some W83 and ATCC 33277 strains. Furthermore, menadione at 14.5 μM frequently inhibited cell growth, while phylloquinone at 145 μM promoted cell growth.
These results indicate that menadione and phylloquinone act as growth factors for ATCC 33277 but that menadione also can inhibit P. gingivalis growth. Thus, we propose that phylloquinone be used instead of menadione in P. gingivalis growth experiments requiring vitamin K.
牙龈卟啉单胞菌是慢性牙周炎的病因。甲萘醌（维生素 K）和叶绿醌（维生素 K）是众所周知的牙龈卟啉单胞菌生长因子，而甲萘醌广泛用于生长实验。在这里，我们试图确定在牙龈卟啉单胞菌生长实验中叶绿醌和甲萘醌的差异，迄今为止，这方面的研究还不够充分。
我们研究了甲萘醌和叶绿醌对两种 W83 株和七种 ATCC 33277 株牙龈卟啉单胞菌生长的影响。
ATCC 33277 株在复杂培养基（营养培养基）中叶绿醌浓度为 2.9 μM 和两种最低培养基中浓度为 29 μM 时生长良好。相比之下，W83 株在三种不同的培养基中生长良好，无需甲萘醌或叶绿醌。2.9 μM 的甲萘醌，通常用于培养牙龈卟啉单胞菌的浓度，支持大多数 ATCC 33277 株的生长，但抑制了一些 W83 和 ATCC 33277 株的生长。此外，14.5 μM 的甲萘醌常抑制细胞生长，而 145 μM 的叶绿醌促进细胞生长。
这些结果表明，甲萘醌和叶绿醌是 ATCC 33277 的生长因子，但甲萘醌也能抑制牙龈卟啉单胞菌的生长。因此，我们建议在需要维生素 K 的牙龈卟啉单胞菌生长实验中使用叶绿醌代替甲萘醌。
